Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution.

By A. Lyons. JIM - Journal of Immunological Methods, 2000

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GADD45β/GADD45γ and MEKK4 comprise a genetic pathway mediating STAT4‐independent IFNγ production in T cells (2004, methodology): “CFSE labeling was performed as described elsewhere (Lyons, 2000).”
Defective dendritic cell migration and activation of adaptive immunity in PI3Kγ‐deficient mice (2004, positive): “Morphology and distribution of green autofluorescent cells as well as their colocalization with anti-MHC-class II cells were evaluated by fluorescence microscopy (Lyons, 2000).”
Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester (2007, background): “The intracellular fluorescent dye, CFSE, has been found to be particularly effective at monitoring lymphocyte division in many experimental situation”