The potential of Raman microscopy and Raman imaging in plant research
By N. Gierlinger, M. Schwanninger. 2007
242 citations recorded by the source corpus.
To gain a better understanding on structure, chemical composition and properties of plant cells, tissues and organs several microscopic, chemical and physical methods have been applied during the last years. However, a knowledge gap exists about the location, quantity and structural arrangement of molecules in the native sample or what happens on the molecular level when samples are chemically or mechanically treated or how they respond to mechanical stress. These questions need to be answered to optimise utilization of plants in food industry and pharmacy and to understand structure-function relationships of plant cells to learn from natures unique. Advances in combining microscopy with Raman spectroscopy have tackled this problem in a non-invasive way and provide chemical and structural information in situ without any staining or complicated sample preparation. In this review the different Raman techniques (e.g. near infrared Fourier Transform Raman spectroscopy (NIR-FT), resonance Raman spectroscopy, surface-enhanced Raman spectroscopy) are briefly described before approaches in plant science are summarised. Investigations on structural cell wall components, valuable plant substances, metabolites and inorganic substances are included with emphasis on Raman imaging. The introduction of the NIR-FT-Raman technique led to many applications on green plant material by eliminating the problem of sample fluorescence. For mapping and imaging of whole plant organs (seeds, fruits, leaves) the lateral resolution (~10 μm) of the NIR-FT technique is adequate, whereas for investigations on the lower hierarchical level of cells and cell walls the high resolution gained with a visible laser based system is needed. Examples on high resolution Raman imaging are given on wood cells, showing that changes in chemistry and orientation can be followed within and between different cell wall layers having dimensions smaller than 1 μm. In addition imaging the distribution of amorphous silica is shown on horsetail tissue, including an area scan from a cross section as well as a depth profiling within a silica rich knob of the outer stem wall.
Subfields: Analytical Chemistry, Biophysics, Food Science
View Paper: https://www.semanticscholar.org/paper/9cbfe895d0e1d92f1ae89aa38a479d5b0ba78dd2
How researchers see this paper
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In Analytical Chemistry
- M. Schwanninger
- Antonio J. Plaza
- Susan L. Ustin
- Jochem Verrelst
- Satoru Tsuchikawa
- Helena Pereira
- A. Lourenço
In Biophysics
In Food Science
Citations in the corpus
- Earth system science related imaging spectroscopy — an assessment (2009, background): “In the future particular attention will be paid to integrating plant physiological approaches from leaf to molecular level (Gierlinger & Schwanninger, 2007; Ustin et al., 2009-in this issue), and optimizing the coupling of canopy scale RTM with atmospheric RTM, supporting large region (semi-)…”
- Lignin Biosynthesis and Structure1 (2010, background): “Lignins can even be dissimilar at the level of individual cell wall layers, as revealed by Raman, IR, and UV microspectroscopy, or by immunolabeling of the secondary wall with antibodies cross-reacting with specific lignin substructures (Shi et al., 2006; Gierlinger and Schwanninger, 2007; Gou et al”
- A Review of Recent Near-Infrared Research for Wood and Paper (Part 2) (2013, background): “Gierlinger and Schwanninger (175) reviewed the potential of NIR-FT-Raman in plant research.”