Characterization of the Physical Interaction between Estrogen Receptor α and JUN Proteins*

By C. Teyssier, K. Belguise, F. Galtier, D. Chalbos. Journal of Biological Chemistry, 2001

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Activated estrogen receptor α (ERα) modulates transcription triggered by the transcription factor activator protein-1 (AP-1), which consists of Jun-Jun homodimers and Jun-Fos heterodimers. Previous studies have demonstrated that the interference occurs without binding of ERα to DNA but probably results from protein·protein interactions. However, involvement of a direct interaction between ERα and AP-1 is still debated. Using glutathioneS-transferase pull-down assays, we demonstrated that ERα bound directly to c-Jun and JunB but not to FOS family members, in a ligand-independent manner. The interaction could occur when c-Jun was bound onto DNA, as shown in a protein-protein-DNA assay. It implicated the C-terminal part of c-Jun and amino acids 259–302 present in the ERα hinge domain. ERα but not an ERα mutant deleted of amino acids 250–303 (ER241G), also associated with c-Jun in intact cells, in the presence of estradiol, as shown by two-hybrid and coimmunoprecipitation assays. We also show that ERα, c-Jun, and the p160 coactivator GRIP1 can form a multiprotein complex in vitro and in intact cells and that the ERα·c-Jun interaction could be crucial for the stability of this complex. VP16-ERα and c-Jun, which both interact with GRIP1, had synergistic effect on GAL4-GRIP1-induced transcription in the presence of estradiol, and this synergistic effect was not observed with the ERα mutant VP16-ER241G or when c-Fos, which bound GRIP1 but not ERα, was used instead of c-Jun. Finally, ER241G was inefficient for regulation of AP-1 activity, and an ERα truncation mutant encompassing the hinge domain had a dominant negative effect on ERα action. These results altogether demonstrate that ERα can bind to c-Jun in vitro and in intact cells and that this interaction, by stabilizing a multiprotein complex containing p160 coactivator, is likely to be involved in estradiol regulation of AP-1 responses.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/9cb372d77cb4665d70103bc7e2c143609e3936be

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