A general method for the covalent labeling of fusion proteins with small molecules in vivo

By Antje Keppler, S. Gendreizig, Thomas Gronemeyer, H. Pick, H. Vogel, Kai Johnsson. Nature Biotechnology, 2003

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Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles (2010, background): “One example is the protein O6-alkylguanine-DNA-alkyltransferase (hAGT) that binds specifically and eventually by a covalent bond to benzylguanine (Keppler et al. 2003; Kufer et al. 2005).”
Propagation of centromeric chromatin requires exit from mitosis (2007, methodology): “The SNAP tag, a self-labeling enzyme enabling in vivo fl uorescent pulse-chase imaging Our effort validates the SNAP tag (Keppler et al., 2003, 2004, 2006), coupled with indirect immunofl uorescence or live-cell imaging, as an approach capable of visualizing and tracking intracellular dynamics of…”
A general method to improve fluorophores for live-cell and single-molecule microscopy (2015, background): “(e) Normalized absorption (abs) and fluorescence emission (fl) spectra for TMR ( ) and JF549 (4).”
Direct stochastic optical reconstruction microscopy with standard fluorescent probes (2011, methodology): “Alternative approaches include the SNAP-tag that allows labeling of fusion proteins of human O 6 -alkylguanine-DNA alkyltransferase with O 6 -benzylguanine derivative”