A critical experimental evaluation of methods for determination of NH4+ in plant tissue, xylem sap and apoplastic fluid

By Søren Husted, C. Hebbern, M. Mattsson, J. Schjoerring. 2000

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The effects of NH4+ and NO3- on growth, resource allocation and nitrogen uptake kinetics of Phragmites australis and Glyceria maxima (2005, unknown): “Although the discrepancy in methods for NH4 + detection (Husted et al., 2000) and species specific differences have to be considered, observed tissue NH4 + concentrations were below the levels usually described as necessary to induce symptoms of NH4 + toxicity (Barker, 1999; Vollbrecht and Kasemir,…”
Nitrogen use efficiency (NUE) in rice links to NH4+ toxicity and futile NH4+ cycling in roots (2013, methodology): “The analytical principle was based on detection of fluorescence upon reaction between the fluorochrome OPA and NH4 + as described by Husted et al. (2000).”
NH4+ toxicity in higher plants: a critical review (2002, background): “…including growth on nitrate (Blackwell et al. 1987, Jackson et al. 1993, Feng et al. 1998), such cellular measurements belie the widely-held notion that free ammonium does not accumulate in plant tissues (Kafkafi and Ganmore-Neumann 1997, Tobin and Yamaya 2001 – but cf. Husted et al. 2000).”
Effects of Ammonium Toxicity on Nitrogen Metabolism and Elemental Profile of Cucumber Plants (2007, methodology): “For derivatization, 6-aminoquinolylN-hydroxysuccinimidyl-carbamate was used according to Van Wandelen and Cohen (1997) and Husted et al. (2000).”