A morphological classification proposal for curimba (Prochilodus lineatus) sperm damages after cryopreservation

By Aléssio Batista Miliorini, L. Murgas, P. V. Rosa, G. Oberlender, Gilmara Junqueira Machado Pereira, D. V. Costa. 2011

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The present study evaluated some cryoprotectants and concentrations for curimba (Prochilodus lineatus) semen preservation from a simple methodology of analysis of sperm morphology. Semen of nine males was diluted (1:4) into eight cryosolutions, all of them containing 5% of BTS TM Minitb, still comprising methanol or dimethyl sulphoxide (DMSO) at four concentrations: 5%,7.5%, 10% and 12.5%. Morpholo- gical sperm analysis was performed on an optical mi- croscope at 1000 magni¢cation. Damages on the head, midpiece and tail rest (the main and terminal portions) were investigated and classi¢ed as primary or secondary, according to the assumed in£uence on fertilization. There was a linear reduction in the total damages with an increase in the cryoprotectant con- centration. Samples with DMSO showed the lowest percentage of fractured tails and tail stumps. The cryopreservation protocol retained the fertilizing po- tential of the sperm after freezing and both methanol 5% and DMSO 7.5% conferred adequate protection to the curimba sperm cells. Sperm morphology, as pointed out here, must be incorporated into routine ¢sh sperm (cryopreserved or not) analysis once sperm defects in£uence fertilization directly. To the best of our knowledge, this was the ¢rst report on curimba sperm damages after cryopreservation by optical microscopy. The classi¢cation model pre- sented in this study can be adequate to tackle ¢sh sperm damages.

Subfields: Genetics, Physiology, Reproductive Medicine

View Paper: https://www.semanticscholar.org/paper/9b43f347153c0ce93a6f6ef51fd67ff140955330