Riboswitches in Eubacteria Sense the Second Messenger Cyclic Di-GMP

By Narasimhan Sudarsan, Elaine R. Lee, Zasha Weinberg, R. H. Moy, Jane N. Kim, Kristian H. Link, Ronald R. Breaker. Science, 2008

721 citations recorded by the source corpus.

Cyclic di-guanosine monophosphate (di-GMP) is a circular RNA dinucleotide that functions as a second messenger in diverse species of bacteria to trigger wide-ranging physiological changes, including cell differentiation, conversion between motile and biofilm lifestyles, and virulence gene expression. However, the mechanisms by which cyclic di-GMP regulates gene expression have remained a mystery. We found that cyclic di-GMP in many bacterial species is sensed by a riboswitch class in messenger RNA that controls the expression of genes involved in numerous fundamental cellular processes. A variety of cyclic di-GMP regulons are revealed, including some riboswitches associated with virulence gene expression, pilus formation, and flagellum biosynthesis. In addition, sequences matching the consensus for cyclic di-GMP riboswitches are present in the genome of a bacteriophage.

Subfields: Ecology, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/9acead497f335c3548bc64e635814f642cd80d7f

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In Ecology

1. Derek R. Lovley
2. Kelly P. Nevin
3. N. Malvankar
4. Amelia-Elena Rotaru
5. Ashley E. Franks
6. P. Shrestha
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8. Tian Zhang

In Genetics

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In Molecular Biology

1. Derek R. Lovley
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Citations in the corpus

• Geobacter: the microbe electric's physiology, ecology, and practical applications. (2011, background): “, 2007), which have been shown to sense c-di-GMP in other bacteria (Sudarsan et al., 2008).”
• Signals, Regulatory Networks, and Materials That Build and Break Bacterial Biofilms (2009, background): “Because high intracellular c-di-GMP levels are known to promote biofilm formation and decrease flagellar gene expression, this riboswitch provides a mechanism for inverse regulation of flagellar gene transcription and biofilm formation (307).”
• Regulatory RNAs in Bacteria (2009, background): “Finally, thermosensors and riboswitches can have roles as regulators of pathogenesis, upregulating virulence genes upon increased temperature encountered in host cells or upon binding signals such as the ''second messenger'' cyclic di-GMP (Johansson et al., 2002; Sudarsan et al., 2008).”
• CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS (2009, background): “Besides a c-di-GMP binding riboswitch controlling translational initiation has been identified (Sudarsan et al., 2008).”
• Biological Insights from Structures of Two-Component Proteins (2009, background): “Some targets of this second messenger have been identified, such as the PilZ c-di-GMP-binding domain (9) and riboswitches (89), but target identification, mechanisms of regulation, and whether the effects of c-di-GMP are global or local are areas of active investigation (91).”
• Riboswitches and the RNA world. (2012, neutral): “2009) and c-di-GMP (Sudarsan et al. 2008).”