Rapid Biosynthesis of Silver Nanoparticles Using Culture Supernatant of Bacteria with Microwave Irradiation

By N. Saifuddin, C. Wong, A. A. N. Yasumira. 2009

658 citations recorded by the source corpus.

The development of rapid and reliable processes for the synthesis of nanosized materials is of great importance in the field of nanotechnology. Synthesis of silver nanoparticles using microorganism have been reported, but the process is rather slow. In this paper, we describe a novel combinatorial synthesis approach which is rapid, simple and “green” for the synthesis of metallic nanostructures of noble metals such as silver (Ag), by using a combination of culture supernatanant of Bacillus subtilis and microwave (MW) irradiation in water in absence of a surfactant or soft template. It was found that exposure of culture supernatanant of Bacillus subtilis and microwave irradiation to silver ion lead to the formation of silver nanoparticles. The silver nanoparticles were in the range of 5-60 nm in dimension. The nanoparticles were examined using UV-Visible Spectroscopy, and Transmission Electron Microscopy (TEM) analyses. The formation of nanoparticles by this method is extremely rapid, requires no toxic chemicals and the nanoparticles are stable for several months. The main conclusion is that the bio-reduction method to produce nanoparticles is a good alternative to the electrochemical methods.

Subfields: Biomedical Engineering, Materials Chemistry

View Paper: https://www.semanticscholar.org/paper/99e5fc4c9aa24b4e3de160be707199cfa194116f

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In Materials Chemistry

Citations in the corpus

Biosynthesis of silver nanoparticles using aqueous extract from the compactin producing fungal strain (2009, methodology): “A combinatorial synthesis approach by using a combination of fungal culture supernatant and microwave irradiation in water can be used as one of the approach for monodisperse nanoparticles biosynthesis [34].”
Synthesis of silver nanoparticles: chemical, physical and biological methods (2014, background): “Saifuddin and coworkers (110) have described a novel combinational synthesis approach for the formation of silver NPs by using a combination of culture supernatant of B.”
Synthesis paradigm and applications of silver nanoparticles (AgNPs), a review (2017, positive): “The chemical stability and catalytic effect of silver nanoparticles have an advantage over the other metallic nanoparticles for antibacterial [2,47], antiviral [44,58], anticancer [75] antifungal and to anti-inflammatory activities [3,4].”
Antibacterial mechanism of biogenic silver nanoparticles of Lactobacillus acidophilus (2015, neutral): “licheniformis,[17] Aspergillus flavus,[20] Bacillus subtilis,[21] Aeromonas sp.”
Honey Mediated Green Synthesis of Nanoparticles: New Era of Safe Nanotechnology (2017, methodology): “Bacteria were used to synthesize several nanoparticles including gold, silver, silver oxide, titanium dioxide, and cadmium sulfide [24–30] while fungi were used to synthesize silver [31–36], titanium dioxide [29], and cadmium sulfide [30].”
Algal Nanoparticles: Synthesis and Biotechnological Potentials (2016, methodology): “So far, several plant extract [11], bacteria [12], fungi [13], enzymes [14], and algae [15] have been used for the synthesis of NPs.”
A review on biosynthesis of silver nanoparticles and their biocidal properties (2018, methodology): “[14] have demonstrated an extracellular biosynthesis of Ag NPs (∼ 5–50 nm) using a combination of culture supernatant of B.”