Modulation of antioxidant enzymes by salicylic acid in arsenic exposed Glycine max L.

By Chandrakar, Amit Dubey, S. Keshavkant. 2016

103 citations recorded by the source corpus.

To investigate the physiological and metabolic attributes of arsenic (As) stress tolerance conferred by exogenous salicylic acid (SA), Glycine max L. (variety JS 335) seeds were aseptically germinated over filter paper moistened with SA (500 µM) and/or10 and 100 µM As (Sodium arsenite was used). On 2nd and 5th days of germination, the growing radicles were harvested, and analyzed for growth and different metabolic attributes. Findings exemplified that As significantly decreased germination percentage, radicle length, dry mass and activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX), while stimulated the contents of As, reactive oxygen species (ROS), lipoxygenase (LOX), guaiacol peroxidase (POD) and proline. Additionally, isozymes of antioxidants were also scrutinized over Native-PAGE gels and were found to be altered considerably under As-stress. However, exogenous SA remarkably enhanced germination percentage, growth indices, activities of SOD, CAT and APX, and proline accumulation along with reduced As, ROS and LOX, and restoring POD in As-stressed seedlings. In conclusion, SA confers As-stress tolerance to Glycine max L. by regulating the antioxidant enzymes and proline accumulation thereby reduced As content and ROS production. Further study is intended, particularly at gene level, to understand precise mechanism(s) involved in SA-mediated As-stress tolerance.

Subfields: Environmental Chemistry, Plant Science

View Paper: https://www.semanticscholar.org/paper/9895b286bdff45c3d254939c63c8a42cd8d550bb

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Plant Science

1. Vinod Kumar
2. M. Shahid
3. R. Bhardwaj

Citations in the corpus

• Phytohormones Regulate Accumulation of Osmolytes Under Abiotic Stress (2019, background): “100, 200, 300 and 400 μM Heavy Metal (As-10, 25, 50, 75, 100, and 125 μM) Remarkable enhancement in proline levels was observed by SA dose [207]”
• Arsenic Uptake, Toxicity, Detoxification, and Speciation in Plants: Physiological, Biochemical, and Molecular Aspects (2018, background): “Proline protects plants against ROS-mediated damages [32,48,233] by serving as a singlet oxygen quencher and OH scavenger, so it stabilizes the structure of proteins, DNA, and cellular membranes [22].”