Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology.

By A. Croxatto, Guy Prod'Hom, Gilbert GREUB. FEMS Microbiology Reviews, 2012

900 citations recorded by the source corpus.

Until recently, microbial identification in clinical diagnostic laboratories has mainly relied on conventional phenotypic and gene sequencing identification techniques. The development of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) devices has revolutionized the routine identification of microorganisms in clinical microbiology laboratories by introducing an easy, rapid, high throughput, low-cost, and efficient identification technique. This technology has been adapted to the constraint of clinical diagnostic laboratories and has the potential to replace and/or complement conventional identification techniques for both bacterial and fungal strains. Using standardized procedures, the resolution of MALDI-TOF MS allows accurate identification at the species level of most Gram-positive and Gram-negative bacterial strains with the exception of a few difficult strains that require more attention and further development of the method. Similarly, the routine identification by MALDI-TOF MS of yeast isolates is reliable and much quicker than conventional techniques. Recent studies have shown that MALDI-TOF MS has also the potential to accurately identify filamentous fungi and dermatophytes, providing that specific standardized procedures are established for these microorganisms. Moreover, MALDI-TOF MS has been used successfully for microbial typing and identification at the subspecies level, demonstrating that this technology is a potential efficient tool for epidemiological studies and for taxonomical classification.

Subfields: Cell Biology, Clinical Biochemistry, Public Health, Environmental and Occupational Health

View Paper: https://www.semanticscholar.org/paper/97e50a4ebe291cc4c0393904600d99ef973f8669

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Clinical Biochemistry

1. Ramanan Laxminarayan
2. Manish Kumar
3. Jugsharan Singh Virdi
4. P. Kanaujia
5. A. Croxatto
6. Neelja Singhal

In Public Health, Environmental and Occupational Health

1. Ramanan Laxminarayan
2. A. Croxatto

Citations in the corpus

• The global threat of antimicrobial resistance: science for intervention (2015, background): “and their use in the detection of resistance profiles to certain classes of antibiotics has provided excellent results [28].”
• How to manage Pseudomonas aeruginosa infections (2018, methodology): “The identification of suspected colonies can be rapidly performed using modern technologies such a matrix-assisted-laser-desorption-ionization time-of-flight mass spectrometry (MALDI-TOF) [60].”
• MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis (2015, background): “Under careful experimental conditions, it has also been shown useful for subtyping methicillinresistant S. aureus strains (Croxatto et al., 2012).”