Measurement of biomarker proteins for point-of-care early detection and monitoring of cancer
By James F. Rusling, Challa V. Kumar, J. Gutkind, V. Patel. In Analysis, 2010
513 citations recorded by the source corpus.
This critical review evaluates progress toward viable point-of-care protein biomarker measurements for cancer detection and diagnostics. The ability to measure panels of specific, selective cancer biomarker proteins in physicians’ surgeries and clinics has the potential to revolutionize cancer detection, monitoring, and therapy. The dream envisions reliable, cheap, automated, technically undemanding devices that can analyze a patient’s serum or saliva in a clinical setting, allowing on-the-spot diagnosis. Existing commercial products for protein assays are reliable in laboratory settings, but have limitations for point-of-care applications. A number of ultrasensitive immunosensors and some arrays have been developed, many based on nanotechnology. Multilabel detection coupled with high capture molecule density in immunosensors and arrays seems to be capable of detecting a wide range of protein concentrations with sensitivity ranging into the sub pg mL−1 level. Multilabel arrays can be designed to detect both high and ultralow abundance proteins in the same sample. However, only a few of the newer ultrasensitive methods have been evaluated with real patient samples, which is key to establishing clinical sensitivity and selectivity.
Subfields: Biomedical Engineering, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/95adc174b330a0174f4674643db7041bdd9f5d20
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In Biomedical Engineering
In Molecular Biology
Citations in the corpus
- Electrochemical Immunosensors for Detection of Cancer Protein Biomarkers (2012, background): “Electrochemical detection strategies employing nanostructured surfaces, nanoparticle labels, and magnetic beads offer new opportunities for highly sensitive protein detection.(4,24) 27 Most of the strategies discussed below employ the sandwich immunoassay approach, in which the sensor surface is fir”