Arabidopsis TFL2/LHP1 Specifically Associates with Genes Marked by Trimethylation of Histone H3 Lysine 27
By F. Turck, F. Roudier, S. Farrona, Marie-Laure Martin-Magniette, Elodie Guillaume, N. Buisine, S. Gagnot, R. Martienssen, G. Coupland, V. Colot. PLoS Genetics, 2007
631 citations recorded by the source corpus.
TERMINAL FLOWER 2/LIKE HETEROCHROMATIN PROTEIN 1 (TFL2/LHP1) is the only Arabidopsis protein with overall sequence similarity to the HETEROCHROMATIN PROTEIN 1 (HP1) family of metazoans and S. pombe. TFL2/LHP1 represses transcription of numerous genes, including the flowering-time genes FLOWERING LOCUS T (FT) and FLOWERING LOCUS C (FLC), as well as the floral organ identity genes AGAMOUS (AG) and APETALA 3 (AP3). These genes are also regulated by proteins of the Polycomb repressive complex 2 (PRC2), and it has been proposed that TFL2/LHP1 represents a potential stabilizing factor of PRC2 activity. Here we show by chromatin immunoprecipitation and hybridization to an Arabidopsis Chromosome 4 tiling array (ChIP-chip) that TFL2/LHP1 associates with hundreds of small domains, almost all of which correspond to genes located within euchromatin. We investigated the chromatin marks to which TFL2/LHP1 binds and show that, in vitro, TFL2/LHP1 binds to histone H3 di- or tri-methylated at lysine 9 (H3K9me2 or H3K9me3), the marks recognized by HP1, and to histone H3 trimethylated at lysine 27 (H3K27me3), the mark deposited by PRC2. However, in vivo TFL2/LHP1 association with chromatin occurs almost exclusively and co-extensively with domains marked by H3K27me3, but not H3K9me2 or -3. Moreover, the distribution of H3K27me3 is unaffected in lhp1 mutant plants, indicating that unlike PRC2 components, TFL2/LHP1 is not involved in the deposition of this mark. Rather, our data suggest that TFL2/LHP1 recognizes specifically H3K27me3 in vivo as part of a mechanism that represses the expression of many genes targeted by PRC2.
View Paper: https://www.semanticscholar.org/paper/9591253cabc7cac4bfe3158d230209016ead37c8
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
- R. Amasino
- Anusha Srikanth
- M. Schmid
- F. Turck
- F. Roudier
- F. Fornara
- Ikhlak Ahmed
- Caroline Bérard
Citations in the corpus
- Regulation of flowering time: all roads lead to Rome (2011, background): “In this context it is interesting to note that recent genome-wide surveys indicate that all flowering time genes but CO are H3K27me3 targets [56–58].”
- Regulation and identity of florigen: FLOWERING LOCUS T moves center stage. (2008, background): “In contrast to FT, TSF expression is not increased in tfl2/lhp1 mutants, although chromatin immunoprecipitation on chip data suggest TFL2/LHP1 is associated with the TSF locus (90).”
- Seasonal and developmental timing of flowering. (2010, background): “LHP1 has been shown to bind H3K27me3 and H3K9me3 in vitro, and, in broad genome studies, to be associated with regions of the Arabidopsis genome enriched in H3K27me3, but not with regions enriched in H3K9me3 (Turck et al., 2007).”
- Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, unknown): “Metal concentrations were measured by using inductively coupled plasma optical emission spectrometry SPS3000 (Seiko, Tokyo, Japan).”
- Integrative epigenomic mapping defines four main chromatin states in Arabidopsis (2011, methodology): “For instance, H3K4me3 and H3K36me2 are detected at the 50- and 30-ends of actively transcribed genes, respectively (Oh et al, 2008; Zhang et al, 2009), while H3K27me3 broadly marks repressed genes (Turck et al, 2007; Zhang et al, 2007; Oh et al, 2008).”