Nonblinking and long-lasting single-molecule fluorescence imaging
By I. Rasnik, S. McKinney, T. Ha. Nature Methods, 2006
701 citations recorded by the source corpus.
Subfields: Biophysics, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/957c21c26f03c12c7895961830873cb1430d66f7
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Biophysics
- X. Zhuang
- M. Bates
- T. Ha
- Philip Tinnefeld
- Joseph D. Puglisi
- Colin Echeverría Aitken
- R. Marshall
- Graham T Dempsey
In Molecular Biology
- Philip Tinnefeld
- X. Zhuang
- M. Bates
- Chamaree de Silva
- N. Walter
- Alexander Johnson-Buck
- Nicole Michelotti
- Anthony J. Manzo
Citations in the corpus
- A BIRD’S EYE VIEW: TRACKING SLOW NANOMETER-SCALE MOVEMENTS OF SINGLE MOLECULAR NANO-ASSEMBLIES (2010, positive): “…gluconic acid and water (Benesch and Benesch, 1953 nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into b-carboxy-cis,cis-muconic acid (Aitken et al., 2008), while the antioxidant Trolox suppresses slow blinking and photobleaching of cyanine dyes (Rasnik et al., 2006).”
- Spatially resolved, highly multiplexed RNA profiling in single cells (2015, neutral): “5 mg/mL glucose oxidase (Sigma-Aldrich, G2133), and 40 mg/mL catalase (Sigma-Aldrich, C30) was flown across the sample (50).”
- An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments. (2008, background): “, however, demonstrated that BME induces slow blinking of Cy5 (28).”
- Photophysics of Fluorescence Probes for Single Molecule Biophysics and Super-Resolution Imaging (2012, positive): “Interestingly, Trolox is similarly efficient in blinking and photobleaching suppression even in the absence of separately added oxidant (27, 39).”
- Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging (2011, positive): “Additional control experiments were performed to assess what fraction of fluorescent signals originated from single fluorophores by imaging adsorbed dye-labeled antibodies under buffer conditions that have been previously shown to drastically reduce fluorophore blinking (TN buffer with GLOX and 1 mM”
- Direct stochastic optical reconstruction microscopy with standard fluorescent probes (2011, background): “5, Cy7 or Alexa Fluor 647, 680 and 700 is performed under identical conditions for all of these fluorophores, but the reversibility of photoswitching is dramatically improved when the oxygen concentration is reduced by application of an enzymatic oxygen scavenging syste”