High-resolution quantitative phase-contrast microscopy by digital holography.
By C. Mann, Lingfeng Yu, C. Lo, Myung K. Kim. Optics Express, 2005
664 citations recorded by the source corpus.
Techniques of digital holography are improved in order to obtain high-resolution, high-fidelity images of quantitative phase-contrast microscopy. In particular, the angular spectrum method of calculating holographic optical field is seen to have significant advantages including tight control of spurious noise components. Holographic phase images are obtained with 0.5 mum diffraction-limited lateral resolution and largely immune from the coherent noise common in other holographic techniques. The phase profile is accurate to about 30 nm of optical thickness. Images of SKOV-3 ovarian cancer cells display intracellular and intranuclear organelles with clarity and quantitative accuracy.
Subfields: Atomic and Molecular Physics, and Optics, Radiation, Structural Biology
View Paper: https://www.semanticscholar.org/paper/957943958bc163cd80242141997528d66601927d
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In Atomic and Molecular Physics, and Optics
In Radiation
In Structural Biology
Citations in the corpus
- Applications of Holography in Fluid Mechanics and Particle Dynamics (2010, methodology): “Provided this refractive index is known, this procedure could be used to measure the thickness of a cell (e.g., Mann et al. 2005, Marquet et al. 2005).”
- Quantitative Phase Imaging (2012, methodology): “In recent years we have been witnessing a significant increase in biological applications targeted by DHM methods with improvements in transverse resolution and signal to noise for label-free cell imaging (Ash, Krzewina, & Kim, 2009; Choi & Lee, 2009; Ferraro et al., 2006; Kemmler et al., 2007; Kemp”
- Principles and techniques of digital holographic microscopy (2010, methodology): “Image plane digital holography has been useful in a number of different application areas, including biological microscopy [47,103], where it is important to be able to monitor the live specimen being imaged.”