Amplification of CRAC current by STIM1 and CRACM1 (Orai1)

By C. Peinelt, M. Vig, D. Koomoa, Andreas Beck, M. Nadler, M. Koblan-Huberson, Annette Lis, A. Fleig, R. Penner, J. Kinet. Nature Cell Biology, 2006

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Handbook of ion channels (2015, positive): “One line of evidence came from studies showing that overexpression of ORAI1 together with STIM1 in HEK293 cells produced large currents [55–57] with characteristics consistent with native CRAC channels including high Ca2+ selectivity, low Cs+ permeability, and a narrow pore [25,55,56].”
The functional network of ion channels in T lymphocytes (2009, background): “Consistent with a requirement for both Stim and Orai working together and showing that other cellular components are not limiting for functional expression, co-expression of Stim + Orai (or STIM1 + Orai1) yielded greatly amplified CRAC current amplitudes with normal biophysical characteristics (78, ”
SOAR and the polybasic STIM1 domains gate and regulate the Orai channels (2009, background): “STIM1 is required for the Orais to function as channel”
Store-Operated Calcium Channels. (2015, positive): “Overexpression of Orai1 with STIM1 in HEK293 cells generated store-operated currents up to 100 times larger than endogenous ICRAC that displayed the ion selectivity, pore dimensions, and pharmacological sensitivity of native CRAC channels (239, 291, 358, 420, 446).”