Sequence, Structure, and Function of Peptide Self-assembled Monolayers
By Ann K. Nowinski, Fang Sun, A. White, A. Keefe, Shaoyi Jiang. 2012
286 citations recorded by the source corpus.
Cysteine is commonly used to attach peptides onto gold surfaces. In this work, we show that the inclusion of an additional linker of four residues in length (-PPPPC) of a rigid, hydrophobic nature, is a better choice for forming peptide self-assembled monolayers (SAMs) with well-ordered structure and high surface density. We compare the structure and function of the nonfouling peptide EKEKEKE-PPPPC-Am with EKEKEKE-C-Am. Circular dichroism (CD), attenuated total internal reflection Fourier transform infrared spectroscopy (ATR-FTIR), and molecular dynamics (MD) results show that EKEKEKE-PPPPC-Am forms a secondary structure while EKEKEKE-C-Am has a random structure. Surface plasmon resonance (SPR) sensor results show that protein adsorption on EKEKEKE-PPPPC-Am/gold is very low with small variation while protein adsorption on EKEKEKE-C-Am/gold is high with large variation. X-ray photoelectron spectroscopy (XPS) results show that both peptides have strong gold-thiol binding onto a gold surface, indicating that their difference in protein adsorption is due to their assembled structures. Further experimental and simulation studies were performed to show that -PPPPC is a better linker than -PC, -PPC, and -PPPC. Finally, we extend EKEKEKE-PPPPC-Am with the cell-binding sequence RGD and demonstrate control over specific vs. non-specific cell adhesion without using poly (ethylene) glycol (PEG). Adding a functional peptide to the nonfouling EK sequence avoids complex chemistries that are used for its connection to synthetic materials.
Subfields: Biomaterials, Electrical and Electronic Engineering, Surfaces, Coatings and Films
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Citations in the corpus
- AlphaFold2 and its applications in the fields of biology and medicine (2023, methodology): “McMullen et al. 160 found from yeast that EKP-GCSF and GCSF exhibits similar binding to its receptor GCSF-R.”
- Screening nonspecific interactions of peptides without background interference. (2013, background): “As published previously, this mixed charge sequence was shown to have ultralow levels of adsorption [6,11].”