Effect of Semen Extenders and Storage Time on Sperm Morphology of Four Chicken Breeds

By A. Siudzińska, E. Łukaszewicz. 2008

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SUMMARY A wide range of extenders are available for storing fowl semen. Three of them (the Lakeextender, the EK extender described by Łukaszewicz, and an extender described by Tselutinet al.) were tested for their suitability to store semen of 4 fowl breeds: Green-Legged Partridge,Black Minorca, White Crested Black Polish, and Italian Partridge. Changes in spermatozoaviability and morphology were evaluated in fresh neat semen and in semen diluted 1:4 andstored for 3, 6, and 24 h at 4°C. During the time of semen storage, a decrease in thenumber of live, morphologically normal spermatozoa and an increase in dead spermatozoaand spermatozoa with bent necks were observed. The EK extender was found to be the mostsuitable regardless of the fowl breed because the number of live spermatozoa did not decreaseas much as in semen diluted with the other extenders. The Lake extender had the least beneficialeffect on stored sperm morphology in every breed. Key words: sperm quality, in vitro storage, semen extender, sperm morphology2008 J. Appl. Poult. Res. 17:101–108doi:10.3382/japr.2007-00048

Subfields: Animal Science and Zoology, Physiology, Reproductive Medicine

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