Genetic Mapping of Loci Associated with Seed Phytic Acid Content in CX1834-1-2 Soybean

By D. Walker, A. Scaboo, V. Pantalone, J. Wilcox, H. Boerma. 2006

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Fine Mapping and Candidate Gene Discovery of the Soybean Mosaic Virus Resistance Gene, Rsv4 (2010, background): “Utilizing the WGS, Saghai Maroof et al. (2009) identifi ed genes encoding multi-drug resistance associated proteins (MRPs) which mapped near previously identifi ed quantitative trait loci controlling seed phytate content in the soybean mutant line, CX1834 (Walker et al., 2006; Gao et al., 2008).”
A modified colorimetric method for phytic acid analysis in soybean (2007, methodology): “The pha1 and pha2 (Oltmans et al., 2004) associated with high Pi level in mapping populations derived from CX1834-1-2 ( Walker et al., 2006 ) also were confirmed to be highly associated with low PA content as determined by the modified colorimetric method in our quantitative trait loci (QTL) mapping”
The Low Phytic Acid Phenotype in Soybean Line CX1834 Is Due to Mutations in Two Homologs of the Maize Low Phytic Acid Gene (2009, methodology): “(2000) suggested that only a single locus was responsible for the low phytic acid phenotype, several genetic studies have implicated two independent recessive loci responsible for the low phytic acid phenotype (Gao et al., 2008; Oltmans et al., 2004; Walker et al., 2006).”