ExtraPEG: A Polyethylene Glycol-Based Method for Enrichment of Extracellular Vesicles
By M. Rider, Stephanie N. Hurwitz, D. Meckes. Scientific Reports, 2016
621 citations recorded by the source corpus.
Initially thought to be a means for cells to eliminate waste, secreted extracellular vesicles, known as exosomes, are now understood to mediate numerous healthy and pathological processes. Though abundant in biological fluids, purifying exosomes has been challenging because their biophysical properties overlap with other secreted cell products. Easy-to-use commercial kits for harvesting exosomes are now widely used, but the relative low-purity and high-cost of the preparations restricts their utility. Here we describe a method for purifying exosomes and other extracellular vesicles by adapting methods for isolating viruses using polyethylene glycol. This technique, called ExtraPEG, enriches exosomes from large volumes of media rapidly and inexpensively using low-speed centrifugation, followed by a single small-volume ultracentrifugation purification step. Total protein and RNA harvested from vesicles is sufficient in quantity and quality for proteomics and sequencing analyses, demonstrating the utility of this method for biomarker discovery and diagnostics. Additionally, confocal microscopy studies suggest that the biological activity of vesicles is not impaired. The ExtraPEG method can be easily adapted to enrich for different vesicle populations, or as an efficient precursor to subsequent purification techniques, providing a means to harvest exosomes from many different biological fluids and for a wide variety of purposes.
Subfields: Cancer Research, Infectious Diseases, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/9215ad511457d9cd97c59e5a78b931168149b6fd
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Citations in the corpus
- Exosome: A Review of Its Classification, Isolation Techniques, Storage, Diagnostic and Targeted Therapy Applications (2020, neutral): “Rider et al. 55 compared three technologies of ultracentrifugation, modified polymer co-precipitation (ExtraPEG) and commercially available commercial kits, and found that the first two methods are better than the commercial method and that ExtraPEG is more cost effective, which surpasses…”
- Exosomes as a new frontier of cancer liquid biopsy (2022, background): “, which could rapidly enrich exosomes and obtain sufficient contents harvested from vesicles for downstream analysis [55].”