Tryptophan-dependent production of indole-3-acetic acid (IAA) affects level of plant growth promotion by Bacillus amyloliquefaciens FZB42.

By E. E. Idris, D. Iglesias, Manuel Talón, Rainer Borriss. Molecular Plant-Microbe Interactions, 2007

691 citations recorded by the source corpus.

Phytohormone-like acting compounds previously have been suggested to be involved in the phytostimulatory action exerted by the plant-beneficial rhizobacterium Bacillus amyloliquefaciens FZB42. Analyses by high-performance liquid chromatography and gas chromatography-mass spectrometry performed with culture filtrates of FZB42 demonstrated the presence of indole-3-acetic acid (IAA), corroborating it as one of the pivotal plant-growth-promoting substances produced by this bacterium. In the presence of 5 mM tryptophan, a fivefold increase in IAA secretion was registered. In addition, in the trp auxotrophic strains E101 (deltatrpBA) and E102 (deltatrpED), and in two other strains bearing knockout mutations in genes probably involved in IAA metabolism, E103 (deltaysnE, putative IAA transacetylase) and E105 (deltayhcX, putative nitrilase), the concentration of IAA in the culture filtrates was diminished. Three of these mutant strains were less efficient in promoting plant growth, indicating that the Trp-dependent synthesis of auxins and plant growth promotion are functionally related in B. amyloliquefaciens.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/91991d693960270cf7d9090fc395d9314e2c923b

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

In Plant Science

Citations in the corpus

Characterization of actinomycetes isolates for plant growth promoting traits and their effects on drought tolerance in maize (2020, background): “The increased amount of IAA produced by these bacteria in the medium used was due to the presence of L-tryptophan, as corroborated by Idris et al. (2007) who revealed that the secretion of IAA can be increased by the addition of tryptophan in medium.”
L-Tryptophan-dependent biosynthesis of indole-3-acetic acid (IAA) improves plant growth and colonization of maize by Burkholderia phytofirmans PsJN (2014, neutral): “Fewer studies have described the role of microbial auxin (IAA) in plant growth promotion by using mutants and molecular tools (Patten and Glick 2002; Idris et al. 2007; Spaepen et al. 2008).”
Mechanisms of biocontrol and plant growth-promoting activity in soil bacterial species of Bacillus and Pseudomonas: a review (2012, background): “So far, we have reviewed some of these mechanisms, which involve reduction of iron (ValenciaCantero et al. 2007), and the synthesis and release of phytostimulating compounds such as IAA, ABA and cytokinins (Idris et al. 2007; Arkhipova et al. 2005).”
Rhizobacterial mediation of plant hormone status (2010, background): “Furthermore, strains of the auxin-producing PGPR Bacillus amyloliquefaciens FZB42 with knockout mutations in genes probably involved in IAA metabolism (putative IAA transacetylase, putative nitrilase) were less efficient in promoting plant growth (Idriss et al., 2007).”
Plant Growth Promoting Rhizobacteria: A Critical Review (2011, background): “The tryptophan increases the production of IAA in Bacillus amyloliquefaciens FZB42 [178].”
Bacillus simplex—A Little Known PGPB with Anti-Fungal Activity—Alters Pea Legume Root Architecture and Nodule Morphology When Coinoculated with Rhizobium leguminosarum bv. viciae (2013, neutral): “amyloliquefaciens [9], B.”
Bacillus species as versatile weapons for plant pathogens: a review (2017, background): “Chemical analysis of supernatant of FZB42 showed the presence of substances capable of reacting with IAA-specific antibodies [57].”