Construction of an intron-containing marker gene: Splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation

By G. Vancanneyt, R. Schmidt, A. O’Connor-Sánchez, Lothar Willmitzer, M. Rocha-Sosa. Molecular and General Genetics MGG, 2004

626 citations recorded by the source corpus.

Subfields: Biotechnology, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/915d70dd12ff9c9ce8d0e0d0c67f5b153775c223

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In Biotechnology

1. S. Abel
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1. L. Navarro
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In Plant Science

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Citations in the corpus

• Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation[OPEN] (2016, background): “, 2000) with an introduced potato LS1 intron (Vancanneyt et al., 1990).”
• Genetic Transformation and Hybridization (2002, positive): “1993) harboring either pME504 carrying the nptII and the uidAintron genes (Vancanneyt et al. 1990), or the PZP plasmid carrying both—nptII and GFP genes were used.”
• Characterisation of regenerants obtained under selective conditions after Agrobacterium-mediated transformation of citrus explants reveals production of silenced and chimeric plants at unexpected high frequencies (2004, background): “From the right border to the left border, the three cassettes were: 1 the neomycin phosphotransferase II gene nptII under the control of the nos promoter and terminator, used as selectable marker, 2 the ‘gene of interest’ under the control of the CaMV 35S promoter, and 3 the -glucuronidase gene gusA”
• Extent, variation and breeding impact of natural cross-fertilization in German winter faba beans using hilum colour as marker (2004, methodology): “1 by inserting the P35S GUS intron cassette (Vancanneyt et al., 1990) into the Hind III site of pTF101.”
• Genetic transformation and regeneration of mature tissues of woody fruit plants bypassing the juvenile stage (2004, methodology): “In other cases, in which embryogenic cells of somatic origin, somatic embryos or vegetatively propagated tissues have been used as starting material for transformation, the explants were juvenile (Fitch et al., 1990; Vardi et al., 1990; Brasileiro et al., 1991; Rugini et al., 1991; Uematsu et al., ”
• Study of the factors influencing Agrobacterium-mediated transformation of pea (Pisum sativum L.) (2000, methodology): “Three A. tumefaciens strains ‐ octopine LBA 4404[pAL 4404] [11], nopaline C58C1[pMP90] [15] and L,L-succinamopine, hypervirulent EHA 105[pEHA105] [12] ‐ were used in conjunction with the binary plasmids p35S GUS INT [ 26 ], pGPTV-KAN, pGPTV-HPT, pGPTV-DHFR and pGPTV-BAR [2].”
• High throughput genetic transformation mediated by Agrobacterium tumefaciens in maize (2002, methodology): “The second intron from the potato ST LS1gene (Vancanneyt et al. 1990) was inserted into the GUS coding region to produce intron-GUS, in order to prevent expression of the gene in Agrobacterium.”
• Plant Cell and Tissue Culture - A Tool in Biotechnology: Basics and Application (2009, background): “This totally eliminates expression in any untransformed system (Vancanneyt et al. 1990).”