What makes the bacteriophage λ Red system useful for genetic engineering: molecular mechanism and biological function
By A. Poteete. 2001
119 citations recorded by the source corpus.
Subfields: Ecology, Genetics, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/915998aa53a525b14cba4c874d02c2fd665cff20
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In Ecology
In Genetics
In Molecular Biology
Citations in the corpus
- A highly efficient recombineering-based method for generating conditional knockout mutations. (2003, background): “Only two Red genes are required for the recombination: exo, which encodes a 5–3 exonuclease (Exo) that acts on the 5 ends of the linear dsDNA fragment to produce 3 single-strand DNA (ssDNA) overhangs, and bet, which encodes a pairing protein (Beta) that binds to the 3 ssDNA overhangs created by Exo ”