Biochemical responses of the ethylene-insensitive Never ripe tomato mutant subjected to cadmium and sodium stresses
By C. C. Monteiro, R. F. Carvalho, P. L. Gratão, G. Carvalho, T. Tezotto, L. O. Médici, L. Peres, R. Azevedo. 2011
160 citations recorded by the source corpus.
Abstract In order to further address the known interaction between ethylene and components of the oxidative system, we have used the ethylene-insensitive Never ripe ( Nr ) tomato ( Solanum lycopersicum L.) mutant, which blocks ethylene responses. The mutant was compared to the control Micro-Tom (MT) cultivar subjected to two stressful situations: 100 mM NaCl and 0.5 mM CdCl 2 . Leaf chlorophyll, lipid peroxidation and antioxidant enzyme activities in roots, leaves and fruits, and Na and Cd accumulation in tissues were determined. Although we verified a similar growth pattern and Na and Cd accumulation for MT and Nr , the mutant exhibited reduced leaf chlorophyll degradation following stress. In roots and leaves, the patterns of catalase (CAT), glutathione reductase (GR), ascorbate peroxidase (APX), guaiacol peroxidase (GPOX), superoxide dismutase (SOD) enzyme activity as well as malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ) production under the stressful conditions tested were very similar between MT and Nr mutant. However, Nr fruits showed increased H 2 O 2 production, reduced and enhanced APX activity in NaCl and CdCl 2 , respectively, and enhanced GPOX in NaCl. Moreover, through non-denaturing PAGE, a similar reduction of SOD I band intensity in both, control MT and Nr mutant, treated with NaCl was observed. In leaves and fruits, a similar SOD activity pattern was observed for all periods, genotypes and treatments. Overall the results indicate that the ethylene signaling associated with NR receptor can modulate the biochemical pathways of oxidative stress in a tissue dependent manner, and that this signaling may be different following Na and Cd exposure.
Subfields: Plant Science
View Paper: https://www.semanticscholar.org/paper/90ef046b1efc7286620bff6a78876b16363799bd
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Citations in the corpus
- Salinity effects on nitrogen metabolism in plants – focusing on the activities of nitrogen metabolizing enzymes: A review (2018, background): “…reduction in chlorophyll content and photosynthesis, an increase in the production of reactive oxygen species (ROS), and all such alterations subsequently lead to decreased plant growth and development (Monteiro et al. 2011; Ahmad et al. 2013; Khan et al. 2014; Acosta-Motos et al. 2017).”
- Cadmium stress related to root-to-shoot communication depends on ethylene and auxin in tomato plants (2017, background): “However, dgt mutant might avoid better the stress imposed by Cd than Nr mutant, because the auxin suppression may decline ethylene production, once it is well known that auxin-stimulated ethylene biosynthesis (Gratão et al., 2009, 2012; Monteiro et al., 2011).”