An efficient protocol for genomic DNA extraction fromCitrus species

By Yunjiang Cheng, Wenwu Guo, H. Yi, X.-M. Pang, Xiuxin Deng. Plant Molecular Biology Reporter, 2003

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Exploitation of SSR, SRAP and CAPS-SNP markers for genetic diversity of Citrus germplasm collection (2011, methodology): “Total genomic DNAwas isolated from fresh leaves following the procedure previously described by Cheng et al. (2003). The quality and concentration of the DNA samples were checked in a UV-1601 spectrophotometer (Shimadzu, Japan) andaportionof theDNAwas diluted to (50ng/ l) for use in SSR, SRAP and CA”
Ready-to-use DNA extracted with a CTAB method adapted for herbarium specimens and mucilaginous plant tissue (2006, background): “A number of protocols (e.g., Porebski et al., 1997; Li et al., 2002; Cheng et al., 2003) have been proposed to extract high-quality DNA free of chemicals and impurities, in particular polysaccharides and contaminants in herbarium tissue.”
Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, unknown): “The AhNRMAP1 ORF without a stop codon and containing Xho I and Bgl II restriction sites was ampliﬁed by PCR using primers 5 # - CTCGAGATGGCAAGCGTTCTTAGACA-3 CTCGAGATGGCAAGCGTTCTTAGACA-3 # and 5 # -AGAT- CTGTTCCGGTAGTGGGATATCAG-3 # .”
Over-expression of the Arabidopsis NPR1 gene in citrus increases resistance to citrus canker (2010, methodology): “Citrus genomic DNA was extracted following the previously described method (Cheng et al. 2003).”