Mouse model of chronic and binge ethanol feeding (the NIAAA model)
By Adeline Bertola, S. Mathews, S. Ki, Hua Wang, B. Gao. Nature Protocols, 2013
1,088 citations recorded by the source corpus.
Chronic alcohol consumption is a leading cause of chronic liver disease worldwide, leading to cirrhosis and hepatocellular carcinoma. Currently, the most widely used model for alcoholic liver injury is ad libitum feeding with the Lieber-DeCarli liquid diet containing ethanol for 4–6 weeks; however, this model, without the addition of a secondary insult, only induces mild steatosis, slight elevation of serum alanine transaminase (ALT) and little or no inflammation. Here we describe a simple mouse model of alcoholic liver injury by chronic ethanol feeding (10-d ad libitum oral feeding with the Lieber-DeCarli ethanol liquid diet) plus a single binge ethanol feeding. This protocol for chronic-plus-single-binge ethanol feeding synergistically induces liver injury, inflammation and fatty liver, which mimics acute-on-chronic alcoholic liver injury in patients. This feeding protocol can also be extended to chronic feeding for longer periods of time up to 8 weeks plus single or multiple binges. Chronic-binge ethanol feeding leads to high blood alcohol levels; thus, this simple model will be very useful for the study of alcoholic liver disease (ALD) and of other organs damaged by alcohol consumption.
Subfields: Endocrinology, Diabetes and Metabolism, Epidemiology, Pathology and Forensic Medicine
View Paper: https://www.semanticscholar.org/paper/9053c7aab10ba955ddbd1218656e06c50564fde1
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In Epidemiology
Citations in the corpus
- Bacteriophage targeting of gut bacterium attenuates alcoholic liver disease (2019, positive): “Female and male mice (age, 9–12 weeks) were placed on a chronic–binge ethanol diet (NIAAA model) as described 15 .”
- Mechanisms underlying microbial-mediated changes in social behavior in mouse models of Autism Spectrum Disorder (2018, positive): “Mice were administered FITC-dextran (4KDa, Sigma Aldrich, 44 mg/100 g of body weight) by oral gavage, as described (Bertola et al., 2013).”