β-Lactam induction of colanic acid gene expression in Escherichia coli
By Frances C. Sailer, B. Meberg, K. Young. 2003
85 citations recorded by the source corpus.
An unexpected observation led us to examine the relationship between β-lactam exposure and synthesis of colonic acid capsular polysaccharide in Escherichia coli. Strains containing a cps-lacZ transcriptional fusion were challenged with antibiotics having various modes of action, and gene expression was detected by a disk-diffusion assay and in broth cultures. The cps genes were induced by a subset of β-lactams but not by agents inhibiting protein synthesis or DNA replication, indicating that cps expression was specific and not due to stresses accompanying cell death or by a general inhibition of peptidoglycan synthesis. A narrow concentration just below the MIC triggered cps expression in liquid culture, suggesting the response may be triggered by near-lethal levels of antibiotic. Because colanic acid is important for maturation of biofilm architecture, antibiotics that increase its synthesis might exacerbate the formation or persistence of biofilms.
Subfields: Genetics, Molecular Biology, Molecular Medicine
View Paper: https://www.semanticscholar.org/paper/8eb7b02d3274e851923dc1363158ae95bc27ea27
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Genetics
In Molecular Biology
In Molecular Medicine
Citations in the corpus
- Biofilm formation and the food industry, a focus on the bacterial outer surface (2010, background): “Other studies reported that exposure of biofilm cells to antibiotics elicited a response resulting in increased synthesis of EPS, resulting in a more proliferous biofilm matrix (Sailer et al. 2003; Bagge et al. 2004).”