Regulation of mitochondrial dehydrogenases by calcium ions.
By R. Denton. Biochimica et Biophysica Acta, 2009
836 citations recorded by the source corpus.
Studies in Bristol in the 1960s and 1970s, led to the recognition that four mitochondrial dehydrogenases are activated by calcium ions. These are FAD-glycerol phosphate dehydrogenase, pyruvate dehydrogenase, NAD-isocitrate dehydrogenase and oxoglutarate dehydrogenase. FAD-glycerol phosphate dehydrogenase is located on the outer surface of the inner mitochondrial membrane and is influenced by changes in cytoplasmic calcium ion concentration. The other three enzymes are located within mitochondria and are regulated by changes in mitochondrial matrix calcium ion concentration. These and subsequent studies on purified enzymes, mitochondria and intact cell preparations have led to the widely accepted view that the activation of these enzymes is important in the stimulation of the respiratory chain and hence ATP supply under conditions of increased ATP demand in many stimulated mammalian cells. The effects of calcium ions on FAD-isocitrate dehydrogenase involve binding to an EF-hand binding motif within this enzyme but the binding sites involved in the effects of calcium ions on the three intramitochondrial dehydrogenases remain to be fully established. It is also emphasised in this article that these three dehydrogenases appear only to be regulated by calcium ions in vertebrates and that this raises some interesting and potentially important developmental issues.
Subfields: Biochemistry, Clinical Biochemistry, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/8e897d92e85ada5df1e98c4166f199b923ded13c
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Citations in the corpus
- Activity-driven local ATP synthesis is required for synaptic function (2014, neutral): “Ca is known as an activator of various dehydrogenases of the TCA cycle and regulates various processes of oxidative phosphorylation in the mitochondria (Balaban, 2009; Denton, 2009; Glancy and Balaban, 2012), suggesting that Ca could be one of the regulators of activity-driven ATP synthesis at nerve”
- Physiological mechanisms of seasonal and rapid cold‐hardening in insects (2013, background): “…pathways important for stress tolerance, including stimulation of mitochondrial energy metabolism by activating mitochondrial dehydrogenase (Denton, 2009; Takeuchi et al ., 2009); activation of glycogen phosphorylase kinase, which in turn activates glycogen phosphorylase and stimulates…”
- Role of Mitochondrial Ca2+ in the Regulation of Cellular Energetics (2012, background): “In an important series of papers by McCormack and Denton (10;71;72), the observations of Ca2+ activity in isolated proteins were validated in intact heart and adipose mitochondria.”