Soil Community Analysis Using DGGE of 16S rDNA Polymerase Chain Reaction Products

By Cindy H. Nakatsu, V. Torsvik, L. Øvreås. 2000

291 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/8e37f2c292034b0574329a4c1504845594193d1e

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

In Molecular Biology

Citations in the corpus

Microbial diversity in soil: ecological theories, the contribution of molecular techniques and the impact of transgenic plants and transgenic microorganisms (2004, unknown): “…from rRNA molecules by reverse transcriptase PCR might give fingerprints of the metabolically active microbial populations (Heuer and Smalla 1997a) and DGGE/TGGE methods are useful in the rapid screening of multiple samples for distinguishing soil microbial communities (Nakatsu et al. 2000).”
Soil Microbial Community Analysis Using Denaturing Gradient Gel Electrophoresis (2007, methodology): “Bacterial communities have been examined by PCR-DGGE from almost every soil environment imaginable, including agricultural soils (Nakatsu et al., 2000), plant rhizospheres (Duineveld et al.”
Microbial interactions in the mycorrhizosphere and their significance for sustainable agriculture. (2004, background): “The advantage of DGGE is that it recognises very small differences in the nucleotide sequence, allowing a description of the community structure expressed as band patterns on a gel.”
Diazotrophic diversity, nifH gene expression and nitrogenase activity in a rice paddy field in Fujian, China (2009, methodology): “Composite soil samples were collected according to Nakatsu et al. (2000) and Smalla et al.”
The ecology of soil decomposition (2003, background): “Targeted PCR amplification from 16S rDNA separated by differential gradient gel electrophoresis (DGGE) is more precise, and the appropriate choice of rDNA segments can provide resolution at various taxonomic levels (Muyzer et al., 1993; Grundmann and Normand, 2000; Nakatsu et al., 2000).”
Functional and molecular responses of soil microbial communities under differing soil management practices (2004, background): “…from complex microbial communities, the major limit of the technique is that in soil ecosystems the number of different genomes is so high that the complexity of rDNA fragments can exceed the resolving power of denaturing gels (Nakatsu et al., 2000; Øvreås and Torsvik, 1998).”
Molecular Techniques to Assess Microbial Community Structure, Function, and Dynamics in the Environment (2011, methodology): “In a microbial community investigation, DGGE was applied to soils collected from different agricultural fields in Norway and the USA that were under different agronomic treatments (crop rotation and tillage) (Nakatsua et al. 2000).”
Archaeal communities in High Arctic wetlands at Spitsbergen, Norway (78°N) as characterized by 16S rRNA gene fingerprinting (2005, methodology): “Extraction and purification of peat community DNA was performed with FastDNA SPIN Kit for soil (BIO101, CA, USA) in combination with a freeze–thaw lysis protocol [25].”