The Human Plasma Proteome
By N. Anderson, N. Anderson. Molecular & Cellular Proteomics, 2002
2,627 citations recorded by the source corpus.
The human plasma proteome holds the promise of a revolution in disease diagnosis and therapeutic monitoring provided that major challenges in proteomics and related disciplines can be addressed. Plasma is not only the primary clinical specimen but also represents the largest and deepest version of the human proteome present in any sample: in addition to the classical “plasma proteins,” it contains all tissue proteins (as leakage markers) plus very numerous distinct immunoglobulin sequences, and it has an extraordinary dynamic range in that more than 10 orders of magnitude in concentration separate albumin and the rarest proteins now measured clinically. Although the restricted dynamic range of conventional proteomic technology (two-dimensional gels and mass spectrometry) has limited its contribution to the list of 289 proteins (tabulated here) that have been reported in plasma to date, very recent advances in multidimensional survey techniques promise at least double this number in the near future. Abundant scientific evidence, from proteomics and other disciplines, suggests that among these are proteins whose abundances and structures change in ways indicative of many, if not most, human diseases. Nevertheless, only a handful of proteins are currently used in routine clinical diagnosis, and the rate of introduction of new protein tests approved by the United States Food and Drug Administration (FDA) has paradoxically declined over the last decade to less than one new protein diagnostic marker per year. We speculate on the reasons behind this large discrepancy between the expectations arising from proteomics and the realities of clinical diagnostics and suggest approaches by which protein-disease associations may be more effectively translated into diagnostic tools in the future.
Subfields: Molecular Biology, Spectroscopy
View Paper: https://www.semanticscholar.org/paper/8db336ebad6e35fe435ccdd4c66e217e789c6485
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In Molecular Biology
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- Michael A. Gillette
- N. Rifai
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In Spectroscopy
Citations in the corpus
- Plasma Proteome Database as a resource for proteomics research: 2014 update (2013, background): “Plasma proteome represents an important subproteome, as it harbors proteins secreted from almost all tissues (1,2).”
- Protein biomarker discovery and validation: the long and uncertain path to clinical utility (2006, background): “Yet despite intensified interest and investment, few novel biomarkers are used in clinical practice, and their rate of introduction is fallin”