Bacterial endophytes from arid land plants regulate endogenous hormone content and promote growth in crop plants: an example of Sphingomonas sp. and Serratia marcescens

By S. Asaf, M. Khan, Abdul Latif Khan, Muhammad Mohsin Waqas, Raheem Shahzad, Ah-Yeong Kim, Sang-mo Kang, In-Jung Lee. 2017

107 citations recorded by the source corpus.

ABSTRACT The objective of the present study was to determine the potential plant growth-promoting action of bacterial endophytes isolated from arid land-dwelling plants under normal conditions. Overall, five bacterial endophytes LK11 (Sphingomonas sp. LK11), TP5 (Bacillus subtilis), MPB5.3 (B. subtilis subsp. Subtilis), S9 (B. subtilis subsp. Subtilis), and TP1 (Serratia marcescens) were evaluated based on morphological characteristics after isolation and purification. Phytohormonal analysis of these endophytes predicted indole acetic acid (IAA) production 12.31 ± 0.45 , 6.8 ± 0.59, and 10.5 ± 1.02 μM/mL in the culture broths of LK11, MPB5.3, and TP1, respectively. Under controlled greenhouse conditions, these endophytes were inoculated into soybean, and their growth-promoting characteristics were compared with those of non-phytohormone-producing endophytes. In terms of plant growth promotion, among IAA-producing endophytes, LK11 and TP1 greatly improved physiological characteristics such as shoot/root length, fresh/dry weight, and chlorophyll contents. However, the non-phytohormone-producing endophytes TP5 and S9 did not show a growth-promoting effect. Based on these results, plants inoculated with LK11 and TP1 along with a control were subjected to endogenous hormonal analysis and showed a significant increase in abscisic acid (457.30–398.55 vs. 205.93 ng/g D.W.) and a decrease in jasmonic acid content (50.07–85.07 vs. 93.90 ng/g D.W.), respectively. Total gibberellin content was found to significantly increase in endophyte-inoculated plants (155.43–146.94 ng/g D.W.) as compared to that in controls (113.76 ng/g D.W.). In summary, bacterial endophytes might be used to enhance crop plant physiological characteristics isolated from arid land-inhabiting plants under normal conditions.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/8d69fa56aa2799b0c09dba292f8671d16f043e90

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Citations in the corpus

Aspergillus niger CSR3 regulates plant endogenous hormones and secondary metabolites by producing gibberellins and indoleacetic acid (2018, background): “…pots containing horticulture soil that had the following nutrient composition of peat moss (10–15%), perlite (35–40%), coco peat (45–50%), zeolite (6–8%), and ∼0.09 mg/g, ∼0.205 mg/g, P2O5 ∼0.35 mg/g, and K2O ∼0.1 mg/g to prepare the microbe-free condition (Khan et al. 2011; Asaf et al. 2017).”
Phytohormones and Beneficial Microbes: Essential Components for Plants to Balance Stress and Fitness (2017, background): “…sp. LK11 and Serratia marcescens TP1 produced 12.31 and 10.5 µM mL−1 of IAA in the culture broths, which stimulated root and shoot growth of soybean through increases in ABA and gibberellin and a decrease in jasmonic acid content compared to levels in the control plants (Asaf et al., 2017).”
Plant growth promoting endophytic fungi Asprgillus fumigatus TS1 and Fusarium proliferatum BRL1 produce gibberellins and regulates plant endogenous hormones (2018, methodology): “The uniconazol treated Waito-C seeds were pre-germinated and transferred to autoclave pots containing horticulture soil as explained previously (Asaf et al. 2017; Khan et al. 2011).”