Microplates-based rheometer for a single living cell
By Nicolas Desprat, A. Guiroy, A. Asnacios. 2006
85 citations recorded by the source corpus.
We developed a new versatile micron-scale rheometer allowing us to measure the creep or the relaxation function (time analysis), as well as to determine the dynamical complex modulus (frequency analysis) of a single living cell. In this setup, a microscopic sample can be stretched or compressed uniaxially between two parallel microplates: one rigid, the other flexible. The flexible microplate is used as a nanonewton force sensor of calibrated stiffness, the force being simply proportional to the plate deflection. An original design of the microplates allows us to achieve an efficient feedback control of either strain or stress applied to the cell. Controlling the flexible plate deflection with a typical precision of less than 200nm, we are able to apply stresses ranging from a few pascals to thousands of pascals with a precision better than 2%. The control of the flexible plate deflexion is achieved by direct imaging of the plate tip on a photosensitive detector mounted on the phototube of an inverted mic...
Subfields: Atomic and Molecular Physics, and Optics, Biomedical Engineering, Cell Biology
View Paper: https://www.semanticscholar.org/paper/8d21beaee234526b0d62a9e42605b8c2ca130b40
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In Cell Biology
Citations in the corpus
- Fractional viscoelastic models for power-law materials. (2020, background): “125mm [24], single collagen fibrils [25]; (b) semi-hard zero-fat cheese [26], pure ice at -35◦C [24], asphalt sealants [27] and single MDCK epithelial cell [28].”