AFLP fingerprinting of sesame (Sesamum indicum L.) cultivars: identification, genetic relationship and comparison of AFLP informativeness parameters
By H. Laurentin, P. Karlovsky. Genetic Resources and Crop Evolution, 2007
95 citations recorded by the source corpus.
Amplified fragments length polymorphism (AFLP) was used to distinguish 20 cultivars of sesame (Sesamum indicum L.) and to elucidate the genetic relationship among these genotypes. The data were also used to estimate the usefulness of parameters currently used to assess the informativeness of molecular markers. A total of 339 markers were obtained using 8 primer combinations. Of the bands, 91% were polymorphic. Five primer combinations were able to distinguish all 20 cultivars used. None of the remaining three primer combinations could distinguish all accessions if used alone, but using all three combinations reduced the probability of a random match to 5 × 10−5. Polymorphic information content (PIC), resolving power (Rp) and marker index (MI) of each primer combination failed to correlate significantly with the number of genotypes resolved. Jaccard’s similarity coefficients ranged from 0.31 to 0.78. Fifteen cultivars were grouped by four UPGMA-clusters supported by bootstrapping values larger than 0.70. The grouping pattern was similar to the grouping generated by principal coordinate analysis. The results demonstrated that AFLP-based fingerprints can be used to identify unequivocally sesame genotypes, which is needed for cultivar identification and for the assessment of the genetic variability of breeding stocks. We recommend to use the number of cultivars identified by a primer combination instead of PIC, Rp and MI; and to calculate the maximal, instead of average probability of identical match by chance in the assessment of the informativeness of a marker for cultivar identification.
Subfields: Plant Science
View Paper: https://www.semanticscholar.org/paper/8c2d88dff1ce9541d0b721541e821c61ab7c9bb5
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In Plant Science
Citations in the corpus
- Data analysis for molecular characterization of plant genetic resources (2009, background): “…and Fernandez et al. (2002) found a linear relationship between the ability of a primer to distinguish genotypes and resolving power (Rp), but not with marker index (MI); however this relationship was not found neither by Laurentin and Karlovsky (2007) nor by the data from Rajora and Rahman (2003).”