A Guide to Simple and Informative Binding Assays
By Thomas D. Pollard. Molecular Biology of the Cell, 2010
426 citations recorded by the source corpus.
The aim of binding assays is to measure interactions between two molecules, such as a protein binding another protein, a small molecule, or a nucleic acid. Hard work is required to prepare reagents, but flaws in the design of many binding experiments limit the information obtained. In particular many experiments fail to measure the affinity of the reactants for each other. This essay describes simple methods to get the most out of valuable reagents in binding experiments.
Subfields: Materials Chemistry, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/8c2138da21274623f54a63c02f3b96c3a12c5d60
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Materials Chemistry
In Molecular Biology
Citations in the corpus
- Stochastic mechano-chemical kinetics of molecular motors: A multidisciplinary enterprise from a physicist’s perspective (2012, methodology): “In order to avoid possible pitfalls, one has to design the careful experiment and use appropriate protocols [2190].”