Raf acts downstream of the EGF receptor to determine dorsoventral polarity during Drosophila oogenesis.

By A. Brand, Norbert Perrimon. 1994

In Drosophila, as in mammalian cells, the Raf serine/threonine kinase appears to act as a common transducer of signals from several different receptor tyrosine kinases. We describe a new role for Raf in Drosophila development, showing that Raf acts in the somatic follicle cells to specify the dorsoventral polarity of the egg. Targeted expression of activated Raf (Rafgof) within follicle cells is sufficient to dorsalize both the eggshell and the embryo, whereas reduced Raf activity ventralizes the eggshell. We show that Raf functions downstream of the EGF receptor to instruct the dorsal follicle cell fate. In this assay, human and Drosophila Rafgof are functionally similar, in that either can induce ventral follicle cells to assume a dorsal fate.

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Citations in the corpus

Genetic dissection of mutual interference between two consecutive learning tasks in Drosophila (2022, background): “Acutely knocking down Raf significantly exacerbated the Pro-I, while acute overexpression of Raf-GOF, which encodes a constitutively active Raf kinase (Brand & Perrimon, 1994), significantly reduced Pro-I.”
Rheb promotes cell growth as a component of the insulin/TOR signalling network (2003, methodology): “To make the UAS- Rheb transgene, a full-length EST of Rheb (GH10361; Invitrogen, Carlsbad, CA) was digested with Xho I/ Bgl II, cloned into pUAS”
GAL4 system in drosophila: A fly geneticist's swiss army knife (2002, unknown): “E-mail: jduffy@bio.indiana.edu Published online 00 Month 2002 in Wiley InterScience (www.interscience.wiley.com) DOI: 10.1002/gene.10150 © 2002 Wiley-Liss, Inc. genesis 34:1–15 (2002) man, 1997; Brand and Perrimon, 1994; Zhou et al., 1997).”
Active Protection: Learning-Activated Raf/MAPK Activity Protects Labile Memory from Rac1-Independent Forgetting. (2018, unknown): “We acutely expressed the transgene UAS-RafGOF, which encodes a constitutively active Raf kinase, which is a classical upstream regulator of MAPK (Brand and Perrimon, 1994).”