GENETIC COMPARISONS OF FRESHLY ISOLATED VERSUS CULTURED SYMBIOTIC DINOFLAGELLATES: IMPLICATIONS FOR EXTRAPOLATING TO THE INTACT SYMBIOSIS

By Scott R. Santos, D. J. Taylor, M. Coffroth. 2001

194 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology, Oceanography

View Paper: https://www.semanticscholar.org/paper/8bd917827e7eac152ddb777a4bee32280a859305

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Citations in the corpus

Molecular diversity of symbiotic algae at the latitudinal margins of their distribution: dinoflagellates of the genus Symbiodinium in corals and sea anemones (2002, background): “…(3) the realisation that the non-infective isolates in the classical laboratory studies on specificity in Symbiodinium symbioses are ‘cryptic’ forms that, although readily culturable from symbioses, have never been described as the dominant alga in symbiosis (LaJeunesse 2001, Santos et al. 2001).”
The Adaptive Hypothesis of Bleaching (2004, background): “An important caveat in discussing diversity is the potential for discrepancy between freshly separated and cultured algal symbionts (e.g., Rowan 1998, Carlos et al. 1999, Toller et al. 2001, LaJeunesse 2002, Santos et al. 2001).”
Stability of an octocoral-algal symbiosis over time and space (2003, methodology): “…genetic techniques have been utilized to elucidate variation within a clade (Baillie et al. 1998, 2000a,b, Carlos et al. 2000, LaJeunesse 2001, Santos et al. 2001); however, these techniques are still too coarse to examine the individual level association between a host genotype and its…”
Biogeographic partitioning and host specialization among foraminiferan dinoflagellate symbionts (Symbiodinium; Dinophyta) (2004, result): “The G+C content of the entire region is approximately 50%, which is consistent with the G+C content of the ITS region previously reported within clade B (Santos et al. 2001).”
Diversity and community structure of symbiotic dinoflagellates from Caribbean coral reefs (2002, background): “Though a majority of the hosts sampled in this study contained only one detectable symbiont type, evidence from cell cultures suggests that other symbiont types are present at low and presently undetectable concentrations in the host and that, when freshly isolated and placed into artificial growth ”
Adaptive bleaching: a general phenomenon (2004, background): “Although the diversity of hosts is well established, identification of symbiotic combinations is complicated by problems with identifying hosts (e.g. Santos et al., 2001) and misapplication of host names (e.g. Parasicyonis sp. of Rodriguez-Lanetty et al. (2002) is Entacmaea quadricolor, and…”
Genetic composition of zooxanthellae between and within colonies of the octocoral Plexaura kuna, based on small subunit rDNA and multilocus DNA fingerprinting (2003, background): “Culturable dinoflagellates, however, may be rare clones or clones that have undergone genomic changes due to selection and/or mutation processes (Rowan et al. 1996; Baillie et al. 1998; Carlos et al. 2000; LaJeunesse 2001; Santos et al. 2001).”
High diversity and host specificity observed among symbiotic dinoflagellates in reef coral communities from Hawaii (2004, background): “…F (LaJeunesse 2001), originally cultured by R. Kinzie, and now maintained in labs around the world, is most probably not the ‘‘true’’ symbiont of M. capitata (= verrucosa) but more likely an opportunistic contaminant from the initial isolation process (c.f. Santos et al. 2001; LaJeunesse 2002).”
COMPARISON OF ENDOSYMBIOTIC AND FREE‐LIVING SYMBIODINIUM (DINOPHYCEAE) DIVERSITY IN A HAWAIIAN REEF ENVIRONMENT 1 (2010, background): “…on Symbiodinium cultures have demonstrated that they undergo daily transitions from the nonmotile (coccoid) to a motile form and spend 3–7 h in the ‘‘water column’’ of the flask (Fitt et al. 1981, Santos et al. 2001, Yacobovitch et al. 2004), such observations are still lacking from the field.”
Flexibility and Specificity in Coral-Algal Symbiosis: Diversity, Ecology, and Biogeography of Symbiodinium (2003, unknown): “…polymorphism (SSCP) analysis of ITS nrDNA (Ulstrup & Van Oppen 2003, Van Oppen 2003, Van Oppen et al. 2001), length heteroplasmy of LSU cprDNA (Santos et al. 2001, 2002a,b, 2003a) and phylogenetic analysis of microsatellite flanking regions (S.R. Santos, T.L. Shearer, A.R. Hannes, M.A.…”
Symbiodinium sp. associations in the gorgonian Pseudopterogorgia elisabethae in the Bahamas: high levels of genetic variability and population structure in symbiotic dinoflagellates (2003, methodology): “Unfortunately, Symbiodinium spp. cultures can be difficult to establish (Schoenberg and Trench 1980a) and may not be representative of the host or population from which they were isolated (Santos et al. 2001; LaJeunesse 2002).”
Most corals may not change their symbionts (2006, methodology): “Considering (1), in some instances zooxanthella clades are cultured from a coral that are not known to associate with that coral in nature (Goulet & Coffroth 1997, Santos et al. 2001, LaJeunesse 2002).”
Bleaching Resistance and the Role of Algal Endosymbionts (2009, methodology): “The investigation of intra-specific levels of genetic diversity is still in its infancy (but see examples using allozymes (Schoenberg and Trench 1980), DNA fingerprinting (Goulet and Coffroth 2003a), random amplified polymorphic DNA (Baillie et al. 2000b) and microsatellites ( Santos et al. 2001, 20”
Symbiodinium tridacnidorum sp. nov., a dinoflagellate common to Indo-Pacific giant clams, and a revised morphological description of Symbiodinium microadriaticum Freudenthal, emended Trench & Blank (2015, background): “38), and not an opportunistic species isolated during the culture process (Santos et al., 2001).”
Symbiont acquisition strategy drives host–symbiont associations in the southern Great Barrier Reef (2008, background): “Santos et al. 2001; LaJeunesse 2002; Diekmann et al. 2003; van Oppen et al. 2005; Thornhill et al. 2007).”
Multiple symbiotic partnerships are common in scleractinian corals, but not in octocorals: Comment on Goulet (2006) (2007, background): “Laboratory cultures of zooxanthellae can become dominated by types of Symbiodinium that are not representative of the dominant symbiont in the host from which they were originally isolated (Santos et al. 2001, LaJeunesse 2002).”