Improvement of artificial feeding in a standard in vitro method for rearing Apis mellifera larvae

By P. Aupinel, D. Fortini, H. Dufour, J. Taséi, Bruno Michaud, François Odoux. 2005

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Cell death localization in situ in laboratory reared honey bee (Apis mellifera L.) larvae treated with pesticides (2011, methodology): “In contrast, honey bee larvae treated with acaricides experienced apoptosis and stress-induced, necrotic cell deletion [10], indicating that these different types of cell death can occur simultaneously after exposure to pesticides [32].”
Standard methods for artificial rearing of Apis mellifera larvae (2013, methodology): “In contrast to the numerous progressive feedings of larvae in a colony, larvae in the laboratory are only fed daily or even less frequently, preferably without influencing survival rate (Aupinel et al., 2005; Kaftanoglu et al., 2010).”
Protocol for the in vitro rearing of honey bee (Apis mellifera L.) workers (2016, methodology): “Furthermore, several recently published studies based upon current protocols (Aupinel et al., 2005, 2007, 2009) noted very high control mortality.”
Standard methods for toxicology research in Apis mellifera (2013, background): “Aupinel et al. (2005) devised a standard in vitro test usable for any research topic on larvae (Crailsheim et al., 2013) and more specifically for brood risk assessment (Aupinel et al, 2007b).”