Adventitions shoot formation on excised leaves of in vitro grown shoots of apple cultivars
By F. Fasolo, R. H. Zimmerman, I. Fordham. Plant Cell Tissue and Organ Culture, 2004
162 citations recorded by the source corpus.
Leaves taken from micropropagated shoots of several apple (Malus domestica Borkh.) cultivars were cultured in vitro on Linsmaier & Skoog (LS) medium or the rice anther culture medium of Chu et al. (N6) containing various concentrations of either benzyladenine (BA) or thidiazuron (TDZ) plus naphthaleneacetic acid (NAA). Of the TDZ concentrations tested, 10 μM was most effective and it was equivalent to, or better than, 22 μM BA for both the percentage of leaves regenerating shoots and number of shoots formed per regenerating leaf in almost every experiment. Lower concentrations of NAA (1.1 and 5.4 μM) gave best results with both BA and TDZ. N6 medium gave consistently better results than LS. Lowering total salt concentration or total N concentration of LS to that of N6 did not improve the response nor did changing the NO3:NH4 ratio. The 3–4 leaves on the most distal part of the shoot were most responsive and tended to form the most adventitious shoots. Placing the leaf cultures in the dark for the first 2–3 weeks of the culture period produced the best results. Optimum results were obtained by culturing leaves from the distal part of the shoot in the dark for 2 weeks on N6 medium containing 10 μM TDZ and 1.1 or 5.4 μM NAA, then moving the cultures to 16 h daylight at a photon flux of 60 μmol s-1m-2.
Subfields: Biotechnology, Cell Biology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/8af9f6e8fffe116197f196a8a0e9ba8425dbb1f1
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Citations in the corpus
- The role of cytokinins in shoot organogenesis in apple (2010, background): “…(1988) McIntosh 22.0 lM BA 8.2 ± 4.6 McIntosh Wijcik 22.0 lM BA 11.4 ± 5.1 Gravenstein 22.0 lM BA 7.3 ± 3.1 M26 22.0 lM BA 16.3 ± 7.2 McIntosh Leaf 10 lM TDZ 9.3 Fasolo et al. (1989) Triple Red Delicious 10 lM TDZ 6.1 Paladino Spur McIntosh 10 lM TDZ 6.3 M26 Leaf 4.4 lM BA [14.0 Predieri and…”
- Micropropagation of apple rootstock M.9 EMLA using bioreactor (2003, background): “In vitro micropropagation from nodal explants, leaves or stem portions of apple cultivars and rootstocks has been reported by several authors (Barbieri and Morini, 1987; Fasolo et al., 1989; James et al., 1988; Welander, 1994; Marga et al., 1997; Caboni et al., 2000).”
- In vitro propagation of Indian Kino (Pterocarpus marsupium Roxb.) using Thidiazuron (2007, background): “…primary (shoot induction) and secondary (shoot elongation) medium was successfully applied to a number of plant species, including Malus domestica (Fasolo et al., 1989), Cajanus cajan (Eapen et al., 1998), Nothapodites foetida (Satheeshkumar and Seeni, 2000), Rosa damascena (Kumar et al., 2001),…”