A rapid in vivo shikimate accumulation assay with excised leaf discs

By D. Shaner, T. Nadler-Hassar, W. Henry, Cliff H. Koger. Weed science, 2005

178 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/8aaf72b75f94c238ea035fa907e5ee6cd98bf847

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In Plant Science

In Pollution

Citations in the corpus

Identification of glyphosate-resistant Italian ryegrass (Lolium multiflorum) in Oregon (2005, methodology): “The effect of glyphosate on shikimic acid accumulation in Italian ryegrass was also determined using leaf segments following the method of Shaner et al. (2005).”
Gene amplification confers glyphosate resistance in Amaranthus palmeri (2009, methodology): “Five plants each of R and S were sampled for one 4-mm leaf disk for in vivo measurement of background absorbance in a leaf disk shikimate assay (39) and one leaf disk for RNA extraction (see below).”
Review: Confirmation of Resistance to Herbicides and Evaluation of Resistance Levels (2013, background): “ALS and EPSPS are most active in meristematic tissues and enzyme activities decrease rapidly as leaves mature (Gerwick et al. 1993; Shaner et al. 2005).”
Role of Translocation as A Mechanism of Resistance to Glyphosate (2009, background): “However, Simarmata et al. (2003) did not find any differences in glyphosate uptake into chloroplast, isolated from GS and resistant rigid ryegrass biotypes, despite a difference in the amount of herbicide translocated out of the treated leaf between GS and GR biotypes.”
Biochemical Markers and Enzyme Assays for Herbicide Mode of Action and Resistance Studies (2015, methodology): “In our laboratory, we selected the Shaner et al. (2005) method because of its relative simplicity and good sensitivity.”
Impact of Genetic Background in Fitness Cost Studies: An Example from Glyphosate-Resistant Palmer Amaranth (2014, methodology): “…amaranth seed collected from Macon County, GA in 2009 were characterized as either glyphosate resistant or susceptible on the basis of a leaf-disc shikimate assay (Shaner et al. 2005) and estimation of EPSPS gene copy number via quantitative polymerase chain reaction (PCR) (details given below).”
Glyphosate Resistance in Tall Waterhemp (Amaranthus tuberculatus) from Mississippi is due to both Altered Target-Site and Nontarget-Site Mechanisms (2013, methodology): “Shikimate assay on tall waterhemp populations was conducted following previously reported protocols (Shaner et al. 2005).”