Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin
By B. Zakeri, J. Fierer, Emrah Çelik, E. Chittock, U. Schwarz-Linek, V. Moy, M. Howarth. Proceedings of the National Academy of Sciences of the United States of America, 2012
1,461 citations recorded by the source corpus.
Subfields: Immunology, Molecular Biology, Radiology, Nuclear Medicine and Imaging
View Paper: https://www.semanticscholar.org/paper/89a422b48fcbc0c1a983e35a5769c28900a0dcde
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In Immunology
- Kim-Marie A. Dam
- S. Ou
- Haoqing Wang
- Zhi Yang
- Michel C. Nussenzweig
- Pamela J. Bjorkman
- Alexander A. Cohen
- Priyanthi N P Gnanapragasam
In Molecular Biology
Citations in the corpus
- Title: Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice (2020, positive): “Two versions of each RBD expression vector were made: one including a C-terminal hexahistidine tag (G-HHHHHH) and SpyTag003 (RGVPHIVMVDAYKRYK) (43) (for coupling to SpyCatcher003-mi3) and one with only a hexahistidine tag (for ELISAs).”
- Construction, characterization, and immunization of nanoparticles that display a diverse array of influenza HA trimers (2020, methodology): “The conjugation involves the formation of an isopeptide bond between a lysine from the SpyCatcher protein and an aspartate from the SpyTag [20].”
- Site-specific biotinylation of purified proteins using BirA (2015, background): “Covalent linkage to peptide tags can now be achieved using split inteins (37,38), sortase (39) and SpyCatcher (40), although they have not yet demonstrated the high sensitivity of detection shown by streptavidin or avidin.”