Robust in vitro affinity maturation strategy based on interface-focused high-throughput mutational scanning.

By Y. Fujino, R. Fujita, K. Wada, K. Fujishige, T. Kanamori, Lindsey Hunt, Y. Shimizu, T. Ueda. 2012

33 citations recorded by the source corpus.

Subfields: Molecular Biology, Radiology, Nuclear Medicine and Imaging

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. Stanley Fields
2. Douglas M. Fowler
3. Jason J. Stephany
4. Ben Lehner
5. G. Diss
6. Claudia Bank
7. Joseph A. Marsh
8. B. Livesey

In Radiology, Nuclear Medicine and Imaging

1. Stanley Fields
2. Jason J. Stephany
3. Douglas M. Fowler

Citations in the corpus

• Deep mutational scanning of an antibody against epidermal growth factor receptor using mammalian cell display and massively parallel pyrosequencing (2013, unknown): “Recently Fujino et al.(28) used NGS to analyze selections from point mutant libraries of a single chain Fab anti-tumor necrosis factor receptor antibody using ribosome display.”
• Measuring the activity of protein variants on a large scale using deep mutational scanning (2014, methodology): “Unlike targeted and systematic mutagenesis, which analyze a relatively small number of variants, random mutagenesis begins with a large library (10 5 –10 11 ) of protein variants and relies on selection for function to winnow the population to a small number of ‘winners’.”