Mechanical stiffness grades metastatic potential in patient tumor cells and in cancer cell lines
By Vinay Swaminathan, K. Mythreye, T. O. O 'brien, A. Berchuck, G. Blobe, R. Superfine, Nc. Cancer Research, 2011
685 citations recorded by the source corpus.
Cancer cells are defined by their ability to invade through the basement membrane, a critical step during metastasis. While increased secretion of proteases, which facilitates degradation of the basement membrane, and alterations in the cytoskeletal architecture of cancer cells have been previously studied, the contribution of the mechanical properties of cells in invasion is unclear. Here we apply a magnetic tweezer system to establish that stiffness of patient tumor cells and cancer cell lines inversely correlates with migration and invasion through three-dimensional basement membranes, a correlation known as a power law. We found that cancer cells with the highest migratory and invasive potential are five times less stiff than cells with the lowest migration and invasion potential. Moreover, decreasing cell stiffness by pharmacological inhibition of myosin II increases invasiveness, while increasing cell stiffness by restoring expression of the metastasis suppressor TβRIII/betaglycan decreases invasiveness. These findings are the first demonstration of the power law relation between the stiffness and the invasiveness of cancer cells and show that mechanical phenotypes can be used to grade the metastatic potential of cell populations with the potential for single cell grading. The measurement of a mechanical phenotype, taking minutes rather than hours needed for invasion assays, is promising as a quantitative diagnostic method and as a discovery tool for therapeutics. By demonstrating that altering stiffness predictably alters invasiveness, our results indicate that pathways regulating these mechanical phenotypes are novel targets for molecular therapy of cancer.
Subfields: Atomic and Molecular Physics, and Optics, Cell Biology
View Paper: https://www.semanticscholar.org/paper/896fd0d5177a372c6d0520e04d753facdd6d7eff
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In Atomic and Molecular Physics, and Optics
In Cell Biology
Citations in the corpus
- Cell Stiffness Is a Biomarker of the Metastatic Potential of Ovarian Cancer Cells (2012, result): “Our findings support earlier predictions that the basis of reduced stiffness associated with cancer [39] and highly invasive cells [40] may be associated with cytoskeletal remodeling.”