I-PpoI and I-CreI homing site sequence degeneracy determined by random mutagenesis and sequential in vitro enrichment.

By G. Argast, K. Stephens, M. Emond, R. Monnat. 1998

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Altered target site specificity variants of the I-PpoI His-Cys box homing endonuclease (2007, background): “We focused initially on 5-bp positions, 3 to 7, and on basepair substitutions at these sites that were known to affect cleavage efficiency on the basis of previous homing site degeneracy, biochemical or functional analyses (24,25,36).”
DNA Deformation Energy as an Indirect Recognition Mechanism in Protein-DNA Interactions (2007, background): “All sequences known to bind to IHF [23], CRP [19], [20], I-CreI [21] , I-PpoI [21], and SRF [22] at high affinities were identified from the available literature (see supplemental data).”
Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site. (2000, background): “I-PpoI tolerates base-pair substitutions at several positions within the homing site, but exhibits a strong preference for AT base-pairs in the central four positions of the homing site ( Argast et al., 1998 ).”