Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA

By Christopher D. Richardson, G. J. Ray, M. DeWitt, Gemma L. Curie, J. Corn. Nature Biotechnology, 2016

1,073 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/88d019773635d2ce3dd4e5114a8285446916d000

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In Molecular Biology

1. Joy Y. Wang
2. Jennifer A. Doudna
3. Feng Zhang
4. Lei S. Qi
5. Rodolphe Barrangou

Citations in the corpus

• CRISPR technology: A decade of genome editing is only the beginning (2023, methodology): “Strategies that have been developed include chemically inhibiting key enzymes in the NHEJ pathway, using single-stranded oligodeoxynucleotide templates (which have been shown to increase HDR efficiency to 60% in human cells for a single-nucleotide substitution) (122), utilizing cell cycle stage cont”
• CRISPR Gene Therapy: Applications, Limitations, and Implications for the Future (2020, background): “Rationally designed ssODN templates with optimized length complementarity have been shown to increase HDR rates up to 60% in human cells for single nucleotide substitution (80).”