Cellular compartmentation of cadmium and zinc in relation to other elements in the hyperaccumulator Arabidopsis halleri

By H. Küpper, E. Lombi, F. Zhao, S. McGrath. Planta, 2000

713 citations recorded by the source corpus.

Abstract. The cellular compartmentation of elements was analysed in the Zn hyperaccumulator Arabidopsis halleri (L.) O'Kane & Al-Shehbaz (=Cardaminopsis halleri) using energy-dispersive X-ray microanalysis of frozen-hydrated tissues. Quantitative data were obtained using oxygen as an internal standard in the analyses of vacuoles, whereas a peak/background ratio method was used for quantification of elements in pollen and dehydrated trichomes. Arabidopsis halleri was found to hyperaccumulate not only Zn but also Cd in the shoot biomass. While large concentrations of Zn and Cd were found in the leaves and roots, flowers contained very little. In roots grown hydroponically, Zn and Cd accumulated in the cell wall of the rhizodermis (root epidermis), mainly due to precipitation of Zn/Cd phosphates. In leaves, the trichomes had by far the largest concentrations of Zn and Cd. Inside the trichomes there was a striking sub-cellular compartmentation, with almost all the Zn and Cd being accumulated in a narrow ring in the trichome base. This distribution pattern was very different from that for Ca and P. The epidermal cells other than trichomes were very small and contained lower concentrations of Zn and Cd than mesophyll cells. In particular, the concentrations of Cd and Zn in the mesophyll cells increased markedly in response to increasing Zn and Cd concentrations in the nutrient solution. This indicates that the mesophyll cells in the leaves of A. halleri are the major storage site for Zn and Cd, and play an important role in their hyperaccumulation.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/88704aee67bf2151bff214c4e82bd29aa1964343

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Citations in the corpus

• Heavy metal hyperaccumulating plants: how and why do they do it? And what makes them so interesting? (2011, background): “The preferential heavy metal detoxification/sequestration do occur in locations, such as epidermis [93–96], trichomes [97] and even cuticle [98], where they do least damage to the photosynthetic machinery.”
• Mechanisms of Plant Resistance to Metal and Metalloid Ions and Potential Biotechnological Applications (2005, background): “Evidence for vacuolar location of metal(loid) ions comes from x-ray emmission studies (Kupper et al., 2000), radioactive tracer studies (Lasat et al., 1998), and recent molecular investigations (Persans et al., 2001).”
• Antioxidant defense system in leaves of Indian mustard (Brassica juncea) and rape (Brassica napus) under cadmium stress (2009, background): “In plant cells, cadmium tends to be stored in the apoplast and in vacuoles, which might contribute to Cd tolerance in hyperaccumulator plants and common crops (Küpper et al. 2000; Boominathan and Doran 2003; Cosio et al. 2005; Ma et al. 2005).”
• Cadmium uptake, localization and detoxification in Zea mays (2005, background): “Also in other plant species apoplast and especially the cell wall appeared to be the main site of Cd accumulation in roots, e.g. in Arabidopsis halleri (Küpper et al. 2000) or in Thlaspi caerulescens (Vázquez et al. 1992a).”
• Evolution of metal tolerance in higher plants (2006, background): “Arabidopsis halleri is the most famous example of metal tolerant species in the Sisymbrieae (ERNST 1968; KUPPER et al. 2000; MACNAIR 2002; BERT et al. 2003; BECHER et al. 2004; DRAGER et al. 2004; PAUWELS et al. 2005; WEBER et al. 2006).”
• New approaches for studying and exploiting an old protuberance, the plant trichome. (2004, background): “…GST Johnson, 1975; Behnke, 1984 Pollinator attraction GST Werker, 2000 Ion and pollutant metal secretion GST Salt et al., 1995 Choi et al., 2001; Kupper et al., 2000 Water retention and decreased desiccation in seeds GST Werker, 2000 Allelopathy GST Macias et al., 1999; Werker, 2000 Assist…”
• Root responses to cadmium in the rhizosphere: a review. (2011, background): “Tissue Cd concentrations gradually decrease from the outer cortical parenchyma to the endodermis (Vázquez et al., 1992, 2007; Küpper et al., 2000).”
• Phytoremediation strategies for soils contaminated with heavy metals: Modifications and future perspectives. (2017, background): “The important locations 497 where metal detoxification preferably are cuticle, epidermis and trichomes (Küpper et al., 2000; 498 Robinson et al., 2003; Bidwell et al., 2004; Ma et al., 2005; Asemaneh et al., 2006; Freeman et 499 al., 2006) with least chances of damage.”
• Opportunities and feasibilities for biotechnological improvement of Zn, Cd or Ni tolerance and accumulation in plants (2011, background): “Küpper et al. (1999) found that the highest concentration of leaf Zn and Cd was present in leaf epidermal cells, which contained four”