The Initiation of Eukaryotic DNA Replication.

By Alessandro Costa, J. Diffley. 2022

144 citations recorded by the source corpus.

DNA replication in eukaryotic cells initiates from large numbers of sites called replication origins. Initiation of replication from these origins must be tightly controlled to ensure the entire genome is precisely duplicated in each cell cycle. This is accomplished through the regulation of the first two steps in replication: loading and activation of the replicative DNA helicase. Here we describe what is known about the mechanism and regulation of these two reactions from a genetic, biochemical, and structural perspective, focusing on recent progress using proteins from budding yeast. Expected final online publication date for the Annual Review of Biochemistry, Volume 91 is June 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

Subfields: Genetics, Molecular Biology

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In Molecular Biology

1. Johannes C. Walter
2. Masato T. Kanemaki
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Citations in the corpus

• In silico protein interaction screening uncovers DONSON’s role in replication initiation (2023, background): “Despite these insights, a molecular understanding of how the pre-LC promotes GINS association with Cdc45-MCM2-7 is still lacking, even in yeast, primarily because of the absence of relevant structural information ( 1 ).”
• Novel role of DONSON in CMG helicase assembly during vertebrate DNA replication initiation (2023, unknown): “The basic mechanism of DNA replication initiation is highly conserved among eukaryotes (Parker et al, 2017; Moiseeva & Bakkenist, 2018; Lin & Prasanth, 2021; Costa & Diffley, 2022; Gillespie & Blow, 2022).”
• DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in C. elegans (2023, unknown): “The initiation of chromosome replication is highly regulated in eukaryotic cells, to ensure that a single copy of each chromosome is produced in every cell cycle (1, 2).”