Lemons: Diversity and Relationships with Selected Citrus Genotypes as Measured with Nuclear Genome Markers

By O. Gulsen, M. Roose. 2001

116 citations recorded by the source corpus.

Inter-simple sequence repeats (ISSR), simple sequence repeats (SSR) and isozymes were used to measure genetic diversity and phylogenetic relationships among 95 Citrus L. accessions including 57 lemons (C. limon (L.) Burm. f.), related taxa, and three proposed ancestral species, C. maxima (Burm.) Merrill (pummelo), C. medica L. (citron), and C. reticulata Blanco (mandarin). The ancestry of lemons and several other suspected hybrids was also studied. Five isozyme and five SSR loci revealed relatively little variation among most lemons, but a high level of variation among the relatively distant Citrus taxa. Eight ISSR primers amplified a total of 103 polymorphic fragments among the 83 accessions. Similarity matrices were calculated and phylogenetic trees derived using unweighted pair-group method, arithmetic average cluster analysis. All lemons, rough lemons, and sweet lemons, as well as some other suspected hybrids, clustered with citrons. Most lemons (68%) had nearly identical marker phenotypes, suggesting they originated from a single clonal parent via a series of mutations. Citrons contributed the largest part of the lemon genome and a major part of the genomes of rough lemons, sweet lemons, and sweet limes. Bands that characterize C. reticulata and C. maxima were detected in lemons, suggesting that these taxa also contributed to the pedigree of lemon.

Subfields: Genetics, Plant Science

View Paper: https://www.semanticscholar.org/paper/872875282e672f599a76c56c74f4ba80ad286294

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

1. Ercan Canıhoş
2. İ. Canan
3. O. Gulsen
4. Ubeyit Seday
5. A. Uzun

In Plant Science

1. Athanasios Tsaftaris
2. Chunyun Hu
3. Xiaoming Pang
4. O. Gulsen
5. Ioannis Ganopoulos
6. Xiuxin Deng
7. A. Uzun

Citations in the corpus

• New universal mitochondrial PCR markers reveal new information on maternal citrus phylogeny (2011, background): “Gulsen and Roose (2001) observed that cpDNA patterns clustered ‘Rangpur’ lime with ‘Sunki’ mandarin but ‘Cleopatra’ and ‘Shekwasha’ were not included in their study.”
• Genetic diversity, structure and fruit trait associations in Greek sweet cherry cultivars using microsatellite based (SSR/ISSR) and morpho-physiological markers (2011, background): “Although they are highly heterozygous, the majority of 57 lemon cultivars remained indistinguishable when analyzed with microsatellite markers whereas better discrimination was achieved by analyzing the same material using ISSRs (Gulsen and Roose 2001).”
• The Origin of Cultivated Citrus as Inferred from Internal Transcribed Spacer and Chloroplast DNA Sequence and Amplified Fragment Length Polymorphism Fingerprints (2010, background): “That mandarin was a parent of rangpur lime was supported by chloroplast data (Gulsen and Roose, 2001a).”
• Phylogenetic relationships within Citrus and its related genera as inferred from AFLP markers (2007, background): “…ISSR (Gulsen and Roose 2001a, b; Fang et al. 1998), organelle genome analysis (Green et al. 1986, Yamamoto et al. 1993), PCR-RFLP analysis of non-coding regions of chloroplast DNA (cpDNA)(Nicolosi et al. 2000; Gulsen and Roose 2001a, b) and sequence analysis of cpDNA region (Araújo et al. 2003).”
• A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers (2010, background): “…gene families and reported to work in a number of grasses as listed by Gulsen et al. (2007); 12 ISSR primers described by Fang and Roose (1997) and Gulsen and Roose (2001)]; 36 RAPD primers (Operon B03, B06, F08, F10, F11, F12, I01, I03, I04, I07, I08, I11, I15, I16, L04, M04, M05, M10, M11, M16,…”