Epigenomic analysis detects widespread gene-body DNA hypomethylation in chronic lymphocytic leukemia
By M. Kulis, S. Heath, M. Bibikova, A. Queirós, A. Navarro, G. Clot, A. Martínez‐Trillos, G. Castellano, I. Brun-Heath, M. Pinyol and 31 more. Nature Genetics, 2012
601 citations recorded by the source corpus.
We have extensively characterized the DNA methylomes of 139 patients with chronic lymphocytic leukemia (CLL) with mutated or unmutated IGHV and of several mature B-cell subpopulations through the use of whole-genome bisulfite sequencing and high-density microarrays. The two molecular subtypes of CLL have differing DNA methylomes that seem to represent epigenetic imprints from distinct normal B-cell subpopulations. DNA hypomethylation in the gene body, targeting mostly enhancer sites, was the most frequent difference between naive and memory B cells and between the two molecular subtypes of CLL and normal B cells. Although DNA methylation and gene expression were poorly correlated, we identified gene-body CpG dinucleotides whose methylation was positively or negatively associated with expression. We have also recognized a DNA methylation signature that distinguishes new clinico-biological subtypes of CLL. We propose an epigenomic scenario in which differential methylation in the gene body may have functional and clinical implications in leukemogenesis.
Subfields: Genetics, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/86c9f0c83b30a4ccdc98ddb5bfc2a3601094b983
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In Genetics
In Molecular Biology
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Citations in the corpus
- Gene Body Methylation can alter Gene Expression and is a Therapeutic Target in Cancer (2014, background): “Recently, more detailed genome-wide studies have also demonstrated that DNA methylation in transcribed regions is correlated with gene expression (Kulis et al., 2012; Maunakea et al., 2010; Varley et al., 2013).”
- Epigenetic Determinants of Cancer. (2016, background): “4) (Jones 2012; Kulis et al. 2012; Shen and Laird 2013).”