A suite of transgenic driver and reporter mouse lines with enhanced brain cell type targeting and functionality

By T. Daigle, L. Madisen, Travis A Hage, Matthew T Valley, Ulf Knoblich, Rylan S. Larsen, Marc M. Takeno, Lawrence Huang, Hong Gu, Rachael Larsen and 31 more. Cell, 2018

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SUMMARY Modern genetic approaches are powerful in providing access to diverse cell types in the brain and facilitating the study of their function. Here we report a large set of driver and reporter transgenic mouse lines, including 23 new driver lines targeting a variety of cortical and subcortical cell populations and 26 new reporter lines expressing an array of molecular tools. In particular, we describe the TIGRE2.0 transgenic platform and introduce Cre-dependent reporter lines that enable optical physiology, optogenetics, and sparse labeling of genetically-defined cell populations. TIGRE2.0 reporters broke the barrier in transgene expression level of single-copy targeted-insertion transgenesis in a wide range of neuronal types, along with additional advantage of a simplified breeding strategy compared to our first-generation TIGRE lines. These novel transgenic lines greatly expand the repertoire of high-precision genetic tools available to effectively identify, monitor, and manipulate distinct cell types in the mouse brain.

Subfields: Biophysics, Cellular and Molecular Neuroscience, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/8694febc1de9bd4f3f71efec5cbd700dc575f21b

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