Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium
By M. Bairu, N. Jain, W. Stirk, K. Doležal, J. van Staden. 2009
99 citations recorded by the source corpus.
Abstract Various experiments were conducted to characterize and control factors affecting shoot-tip necrosis (STN) in Harpagophytum procumbens . Higher cytokinin concentrations increased the incidence of STN and the problem was aggravated by the addition of auxin (IAA) to the multiplication medium. Optimum shoot multiplication was achieved by omitting auxin and using the cytokinin meta -Topolin riboside ( m TR). In the presence of auxin, plantlets produced basal callus that interfered with rooting. The quantity of this basal callus was minimal when m TR was used. Increasing the concentration of either calcium or boron prevented the development of necrotic shoots. When the concentration of both elements was increased simultaneously, negative effects on both growth and STN were observed. Using 6 mM Ca in half-strength MS medium was optimum. Boron was toxic at higher (0.4 and 0.5 mM) concentrations. Plantlets rooted readily in half-strength cytokinin-free MS media supplemented with 2.5 µM IAA. Rooted plantlets produced, using the optimized protocol, were acclimatized successfully by direct transfer to the greenhouse in a 1:1 ratio of sand:soil mixture.
Subfields: Molecular Biology, Plant Science
View Paper: https://www.semanticscholar.org/paper/85783600dfd1526381858b129d884c668f7e3367
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Citations in the corpus
- Factors contributing to in vitro shoot-tip necrosis and their physiological interactions (2009, background): “Minor STN symptoms were also reversed with the exception of plantlets derived from BA-treated medium that failed to reverse minor STN symptoms (Bairu et al. 2009).”
- The role of meta-topolins in alleviating micropropagation problems (2011, background): “Similar observations with respect to BA toxicity have been reported by many researchers for different plant species (Kubaláková and Strnad 1992; Bogaert et al. 2006; Bairu et al. 2007, 2008, 2009).”
- Plant tissue culture: Current status, opportunities and challenges (2010, background): “Hyperhydricity is also linked to shoot-tip necrosis, a physiological disorder showed by in vitro plants as a consequence of high relative humidity, transpiration rate, Calcium availability in the medium and plant growth regulators (Bairu et al., 2009).”