Microbial conversion of choline to trimethylamine requires a glycyl radical enzyme

By Smaranda Crăciun, E. Balskus. Proceedings of the National Academy of Sciences of the United States of America, 2012

672 citations recorded by the source corpus.

Subfields: Molecular Biology, Renewable Energy, Sustainability and the Environment

View Paper: https://www.semanticscholar.org/paper/846aa886dec8670fe19f9b02d80531016df6f3cf

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In Molecular Biology

In Renewable Energy, Sustainability and the Environment

Citations in the corpus

Non-lethal inhibition of gut microbial trimethylamine production for the treatment of atherosclerosis (2015, background): “…E. coli transformed with the cutC/D gene complex from either P. mirabilis or D. alaskensis was supported in both cases by control studies in which the respective cutC gene was mutated to replace previously reported (Craciun and Balskus , 2012) critical active-site residues involved in catalysis ).”
Systems genetics approaches to understand complex traits (2013, background): “A subsequent study identified the enzyme that can generate trimethylamine and showed that it occurs in only certain genera of bacteria 86 .”
Fat, Fiber and Cancer Risk in African Americans and Rural Africans (2015, positive): “This is highly significant as recent studies have shown that choline is extensively metabolized by the microbiota to trimethylamine 20 which is absorbed and metabolized by the liver to trimethylamine-N -oxide (TMAO) which is strongly atherogenic 21 , providing yet another link between westernized…”
Intestinal Microbiota Composition Modulates Choline Bioavailability from Diet and Accumulation of the Proatherogenic Metabolite Trimethylamine-N-Oxide (2015, methodology): “This cluster includes cutC, which encodes a glycyl radical enzyme with choline trimethylamine-lyase activity; cutD, which encodes a glycyl radical-activating protein; and genes encoding proteins involved in the assembly of microcompartments which may sequester the acetaldehyde generated as a by-prod”
DRAM for distilling microbial metabolism to automate the curation of microbiome function (2020, background): “Beyond the content in databases, it is our hope that DRAM can ease the dissemination of emerging metabolisms and biochemistry, offering a community resource to rapidly assimilate these new or refined annotations (Materials and Methods), which currently have very limited, and not rapid, incorporation”